Contributors:Susanne M. Krämer, Ignacia Fuentes, Maria Joao Yubero, Carolina Encina, Jose Farfan, Ignacio Araya, Jimena Castillo, Constanza Fuentes, María Elena McNab, Gisela Zillmann, Marcelo Valle, Anne W. Lucky, Francis Palisson
This Dataset includes following supplementary information for the article "Absence of tongue papillae as a clinical criterion for the diagnosis of Generalized Recessive Dystrophic Epidermolysis Bullosa types."
Supplementary Table 1. Distribution of Absence of Tongue Papillae in Different EB subtypes.
* Patients with normal tongue papillae might have well-defined ulcers, which are different from areas of depapillation.
Supplementary Table 2. Inclusion and Exclusion criteria
Supplementary Figure 1: Patient Flow Diagram.
Recent studies highlight the importance of the temporal domain in visual processing. Critical Flicker Frequency (CFF), the frequency at which a flickering light is perceived as continuous, is a widely used measure for evaluating visual temporal processing. Another important issue to investigate is the cortical interactions arising between the flicker stimuli of both eyes.
This data presents a robust and reliable dichoptic tool for evaluating the CFF threshold in both eyes. This system is based on an analog output device used to independently drive two LEDs through a custom-written MATLAB code (using a laptop PC) for eliciting sinusoidal flickering stimuli and for psychophysically measuring the perceived CFF threshold. The luminance and phases of each LED are individually controlled, enabling the investigation of the effect of phase and luminance differences on binocular summation in subjects with different ocular pathologies. Experiments were designed to evaluate the CFF threshold through a psychophysical test, based on a discrimination task with a stimulus duration of 1 sec, based on a temporal alternative forced-choice paradigm. The target stimulus temporal features were modulated using the staircase method. Subjects were requested to discriminate between a target stimulus (a flickering light at various frequencies) and a flickering light at a frequency of 120Hz, which is significantly higher than the CFF in humans; therefore, it is perceived as constant.
One of the main advantages of the introduced dichoptic presentation system is that it enables the visual temporal performance to be measured under both monocular and binocular conditions where phenomena such as temporal binocular summation (BS) can be evaluated. Moreover, the system offers great flexibility by introducing a stimulus phase shift, which enables studying how stimulus timing affects the temporal function at millisecond scale resolution.
Here we provide all necessary computer code that will enable an easy and quick adaptation of the method by scientists interested in studying the temporal resolution of the visual system in general, and in studying inter-ocular differences or interactions in particular.
Animal modelling of infectious diseases is critical to understand the biology of the pathogens including viruses and to develop therapeutic strategies against it. Herein, we present the sequence homology and expression data analysis of proteins found in Drosophila melanogaster that are orthologous to human proteins, reported to interact with SARS-CoV-2 proteins. The dataset enlists 348 proteins in D.melanogaster that is potentially orthologous to 417 human proteins that interact with 28 proteins encoded by SARS-CoV-2 genome. Expression of D.melanogaster orthologous genes in 26 anatomical positions are also plotted as heat maps in 26 sets, corresponding to the potential protein interactions for each viral protein. The data could be further used to direct experiments for dissecting the biological roles of SARS-CoV-2 proteins in a convenient animal model system like D.melanogaster.
Raw data for the article: Comparative study of the production of soluble factors in human placenta-derived mesenchymal stromal/stem cells grown in adherent conditions or as aggregates in a catheter-like device.
Contributors:Adolf Krige, Jakub Haluska, Paul Christakopoulos, Ulrika Rova
Abstract: Due to the high cost of bioprinters they are not feasible for proof of concept experiments or educational purposes. Furthermore, the more affordable DIY methods all disable the plastic printing capability of the original printer. Here we present an affordable bio-printing modification that is easy to install and maintains the original capabilities of the printer. The modification used mostly 3D printed parts and is based on the popular, open-source Prusa i3 3D printer. The modifications are kept as simple as possible and uses standard slicing software, allowing for installation by less experienced builders. By using disposable syringes and easily sterilizable parts, an aseptic bioprinting setup can be achieved, depending on the environment. It also allows for 2 component printing as well as UV curing.
This directory contains the following datasets and supplementary materials:
The analysis method of the proposed LVRT strategy and the detailed designation process.
The simulation parameters.