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DTSSP and then digested with chymotrypsin and analysed by LC/MS-MS by the University of Cambridge Proteomics Facility. TolC was identified with an emPAI score of 11.1 and AcrA with a score of 10.2. Controls with bovine serum albumin (BSA) were also analysed from the same gel, selecting a band that migrated as a dimer. The crosslinked BSA sample identified 126 peptides that had reacted with the DTSSP, while the control showed 4 false positives. The location of the peptide fragments with mass corresponding to reduced DTSSP were mapped onto the crystal structures of TolC and AcrA and correspond to the equatorial domain and helical hairpin of TolC and the helical hairpin, lipoyl domain and membrane proximal domain of AcrA
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Deliverable 4.1 provides a roadmap of activities for the seven national hubs of the project. These hubs, called Rethinkerspaces, will generate a deep and wide overview of ‘their’ national science communication landscape and act as testbeds and validation mechanisms for the research results of the project. Via their local communities, the Rethinkerspaces will be in charge of creating communities of inquiry to acquire insights into the emerging science communication landscape, map networks, actors, roles and repertoires, contribute to understand sensemaking practices and test a new quality of interactions framework. Besides, they will experiment with new strategies and train other actors in new ways of science communication. The Rethinkerspaces, based in seven European countries (Italy (ZML), the Netherlands (VU), Poland (CSC), Portugal (ITQB NOVA), Serbia (CPN), Sweden (V&A) and the United Kingdom (UWE), will become a learning environment to engage in a collective process of inquiry, experimentation and reflective learning.
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Diversity and complexity of the large surface protein family in the compacted genomes of various Pneumocystis species
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Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multi-copy gene family, termed the major surface glycoprotein (Msg) gene superfamily. This superfamily has been identified in all Pneumocystis species characterized to date, highlighting its important role in Pneumocystis biology. In this report, through a comprehensive and in-depth characterization of 459 msg genes from 7 Pneumocystis species, we demonstrate, for the first time, the phylogeny and evolution of conserved domains in Msg proteins, and provide detailed description of the classification, unique characteristics and phylogenetic relatedness of five Msg families. We further describe the relative expression levels of individual msg families in two rodent Pneumocystis species, the substantial variability of the msg repertoires in P. carinii from laboratory and wild rats, and the distinct features of the expression site for the classic msg genes in Pneumocystis from 8 mammalian host species. Our analysis suggests a wide variety of functions for this superfamily rather than just conferring antigenic variation to allow immune evasion as previously believed. This study provides a rich source of information that lays the foundation for the continued experimental exploration of the functions of the Msg superfamily in Pneumocystis biology.
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The sequence of phase transformations during the crystallization of SrBaFeMoO6–δ by the solid-phase technique from a stoichiometric mixture of simple oxides SrCO3 + BaCO3 + 0.5Fe2O3 + MoO3was studied. It has been established that the synthesis of barium – strontium ferromolybdate proceeds through a series of sequential - parallel stages. It was found that to minimize the effect of intermediate reaction products, it is necessary to use combined synthesis modes. As a result of using combined synthesis modes for annealing for 20 hours and T = 1443 K in vacuum of 10-5Torr at the pressure of residual oxygen gas 10-8 Pa, it was possible to obtain a single-phase barium – strontium ferromolybdate compound with superstructural ordering of iron and molybdenum cations.
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The overall objective of FORESEE, as an H2020 project inside the resilience to extreme events topic (natural, climate change and man-made), is to provide cost effective and reliable tools to improve the resilience of transport infrastructure networks, as the ability to reduce the probability of occurrence, magnitude and/or duration of possible disruptive events that may affect the security and/or the quality of the services provided by infrastructure operators. FORESEE will address through new innovative technologies, methodologies and resilient schemes the effectiveness of resilient measures to improve the ability to anticipate, absorb, adapt to, and/or rapidly recover from a potentially disruptive event.
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This paper is part of a report on community empowerment activities by a team from Padang State University with the focus of the activity being the empowerment of poor housewives through optimizing the function of the plot of land with economically valuable plants, located in Nagari Sungai Buluh Timur, Batang Anai District, Padang Pariaman District. The purpose of the empowerment program is to improve the behavior of poor housewives in order to be able to optimize the utilization of the potential of family resources including specifically the plots of land so that they can increase family income. This dedication activity took the form of counseling with lectures and discussions about the family and its problems, counseling, and workshops on the cultivation of garden plants. Overall Empowerment activities can go well according to plan. Participants were enthusiastic about optimizing the use of the yard, and there was support from the wider community and the village government for the sustainability of the program in the future.
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RAGTIME governance management tool is a risk-based approach for multimodal Transport Infrastructure Asset Management. The governance tool implements a whole system planning software platform able to facilitate a holistic management throughout the entire lifecycle of infrastructures. The development is based on multi-scale data model that uses a risk-based approach, incorporating resilient concepts and mitigation actions to support infrastructure stakeholders during its whole life-cycle by fostering an electronic Tender Process procurement mechanism that promotes the transparency and efficiency, while reducing the risks of the process.
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Sr2FeMoO6–x polycrystalline samples with different oxygen content (6-x) and various degrees of superstructural ordering of Fe/Mo cations (P) were obtained by the solid-phase method from the SrFeO2.52 and SrMoO4 precursors. From the investigation on the influence of oxygen non-stoichiometry and the P parameter on the magnetic properties of Sr2FeMoO6–x, it was found that with an increase in P and a decrease in the (6 - x) value from 5.99 to 5.94, an increase in the magnetization values is observed in the temperature range 77–600 K. For all the Sr2FeMoO6–xsamples there is a tendency that P value rises with increasing x, where, accordingly, the volume fraction of regions in which there are no anti-structural defects increases as well. This is also indicated by Mössbauer spectroscopy data, confirming an increase in the area of the S1 sextet corresponding to Fe ions in highly ordered regions and a decrease in the area of the S2 sextet associated with disordered regions. Using the temperature scanning method, the temperatures of the onset and completion of the transition from the paramagnetic to the ferrimagnetic state and, correspondingly, the blurring width of the transition have been estimated. It turned out that with decreasing P, the blurring of the transition increases, which is associated with an increase in the concentration of anti-structural defects.
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Monitoring data for the three test sites CLIWAT1, CLIWAT2 and goCAM1
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