Contributors:Shaon Basu, Sebastian Mackowiak, Henri Niskanen, Dora Knezevic, Vahid Asimi, Denes Hnisz
These are companion data to the paper "Unblending of transcriptional condensates in human repeat expansion disease" at CELL 2020, May 7, doi: https://doi.org/10.1016/j.cell.2020.04.018
Shaon Basu, Sebastian D. Mackowiak, Henri Niskanen, Dora Knezevic , Vahid Asimi,
Stefanie Grosswendt, Hylkje Geertsema, Salaheddine Ali, Ivana Jerković, Helge Ewers,
Stefan Mundlos, Alexander Meissner, Daniel M. Ibrahim, Denes Hnisz
It includes raw data, microscopy images, computational datasets to generate the figures in the study.
Programming code is available at https://github.com/hniszlab/hoxd13
GEO data is available GSE128818
Contributors:Roberta Cysneiros, Ana Miriã Pacifico, Samuel Batista, Pedro dos Santos, Fernanda Teixeira, Gabriel Silveira, Geraldo Barbosa, Bruna Nascimento, Eduardo Dias-Junior, Sergio Gomes da Silva, Miriam Ribeiro
Early-life status epilepticus produces a high-functioning autism-like phenotype: deficit in social interaction and vocalization, enhanced anxiety, no cognitive impairment and altered functional connectivity within the hippocampus (CA3-CA1) and between the hippocampus and prefrontal cortex. In this study, we used a social memory paradigm to evaluate social memory and social motivation, high demand cognitive tasks (Barnes and Octagonal mazes) to evaluate working memory, reference memory and cognitive flexibility followed by gene and protein expression of molecules related to social behavior, reward system and synapse function. At postnatal day 9, male Wistar rats were subjected to pilocarpine-induced early-life status epilepticus (380mg/kg, i.p.) and the control group received 0.9% saline (0.1 ml/10 g). In adulthood, animals were probed to social recognition memory and cognitive function followed by gene and protein expressions in hypothalamus, hippocampus, amygdala and striatum. In social recognition paradigm, experimental animals spent lower time interacting with the same conspecific, with no enhancement as the familiar animal was replaced by a novel one. In training phase of Barnes maze, the latency to get into the escape cage did not differ between groups. In test phase, as the escape cage has been removed, the time to reach the escape hole did not differ between groups, but the experimental group distributed more evenly the time exploring the peripheral zones the maze, rather than the control group that stayed next to the target hole. In training (TRP) and test phases (TP) of the octagonal maze task, both groups spent similar time to complete the task, but the experimental group exhibited fewer working memory errors during TP with no difference in reference memory errors. The gene expression for oxytocin, oxytocin receptors, dopamine receptors (DR1 and DR2), synapsin and NT3 did not change in experimental group that exhibited higher level of oxytocin in hypothalamus and lower expression of its receptor in hippocampus.
Keeping in mind the earlier argument, there are recognized laws existing in India on several methods of winding up. The principal fact of this research paper is to learning the laws and procedures of voluntary winding up under the Companies Act, 2013. This paper also challenges to analyse and relate the various requirements of winding under the under different countries Legislations.
In the later part, the paper researches deep into making a relative study of the provisions laid down in the Companies Act, 2013 and thus attempts to scrap out the difference with the prevailing provisions under the Act. Further, this paper challenges to conclude by determining the precise variances and similarities in the two laws. This paper also includes the comparison study of how winding up procedure is different in companies Act in comparison to IBC, 2016
Contributors:Delf Kah, Alexander Winterl, Magdalena Přechová, Ulrike Schöler, Werner Schneider, Oliver Friedrich, Martin Gregor, Ben Fabry
Cells in the lungs, the heart, and numerous other organs, are constantly exposed to dynamic forces and deformations. To mimic these dynamic mechanical loading conditions and to study the resulting cellular responses such as morphological changes or the activation of biochemical signaling pathways, cells are typically seeded on flexible 2D substrates that are uniaxially or biaxially stretched. Here, we present an open-source cell stretcher built from parts of an Anet A8 3D printer. The cell stretcher is controlled by a fully programmable open-source software using GCode and Python. Up to 6 flexible optically clear substrates can be stretched simultaneously, allowing for comparative multi-batch biological studies including microscopic image analysis. The cell yield from the cell culture area of 4 cm2 per dish is sufficient for Western-blot protein analysis. As a proof-of-concept, we study the activation of the Yes-associated protein (YAP) mechanotransduction pathway in response to increased cytoskeletal tension induced by uniaxial stretching of epithelial cells. Our data support the previously observed activation of the YAP transcription pathway by stretch-induced increase of cytoskeletal tension and demonstrate the suitability of the cell stretcher to study complex mechano-biological processes.
• Acryl3MM.pdf: Ready-to-cut file for the acrylic glass parts needed to build the electronics and controller unit housing and the cell substrate molds.
• Acryl6MM.pdf: Ready-to-cut file for the acrylic glass parts needed to build the stretcher unit.
• MoldFrame.stl: Ready-to-print file for the 3D printed mold frame.
• Clamp.stl: Ready-to-print file for the 3D printed stretching clamps.
• Stretcher.zip: Customized version of the Raspbian operating version for the Raspberry Pi.
• Raspberry_Pi_Imager_v1.2.zip: Open-source software for Windows PCs to install the Raspberry Pi operating system on a memory card.
• Skynet_for_Stretcher.zip: Modified version of the SkyNet3D (Marlin) open-source firmware for Anet 3D printers.
• Arduino-1.8.0.zip: Modified version of the Arduino integrated development environment for Windows PCs with a pre-installed driver for uploading software to the ANET A8 mainboard.
Capillary origami is used for printing nanoparticles on folded surfaces and the data set presented here shows the results of the coated surfaces. File contains optical images as well as images obtained under the SEM. It also provides particle distribution analysis results.
Therefore 15experimental bench blasts are performed at Wahola limestone quarry with different explosive ratios and drilling parameters to study the results of ground vibration. The objective of this study is to control ground vibration for quarry safety management. During this study ground vibration of experimental blasts is recorded to study their relation with quantity of explosives, ratios of explosives and drilling parameters. To achieve this objective, data is carefully checked and recorded for all important parameters of experimental blasts. Ground vibration level for each blast is recorded separately by using micro mate. On the basis of results of these experimental blasts, new blasting parameters are suggested to control environmental hazards. Explosive for experimental blast was calculated very carefully. All possible hazards were identified and mitigation measures were taken before charging of explosive.
The data shows the Learning Results of students by studying the factors of the English Initial Knowledge and English Achievement Motivation in English Narrative Essay Writing measured by using Analytic Rubric and Holistic Rubric.
The research methodology used was a quasi-experiment with 2 x 2 factorial design which consisted of the Initial Knowledge (A) and the Achievement Motivation factors (B) as independent variables, which are dichotomous variables having two levels: 1 and 2. The Learning Results were scored by Analytical Rubric (Y1) and Holistic Rubric (Y2) as dependent variables. The analysis of the research is by 1) Descriptive Statistics & Test (Test for Equality of Means) and 2) Multivariate Analysis of Variance with 1% level of significance. The population is the first semester students of Academic Year 2014-2015 of The London School of Public Relations, Jakarta while the samples were taken from 2 classes of the lowest and 2 classes of the highest levels of English based on the LSPR English entry test. The researcher developed an Achievement Motivation questionnaire that was distributed to the samples, from which 112 respondents' answers were collected.
The Testing of Hypothesis 1
The mean scores of the bivariate Analytical Rubric and Holistic Rubric have a significant difference between the students having High Initial Knowledge and Low Initial Knowledge.
Based on the Chi-square statistics of 〖 χ〗_0^2 = 326.3242, with df = 2 and p-value = 0.0000 0.10. So, the data does not support the hypothesis.
The Testing Hypothesis 3
The factors of Initial Knowledge and Achievement Motivation together have a significant joint effects on the bivariate Learning Result as assessed by the Analytical Rubric and Holistic Rubric.
Based on the Chi-Square Test statistic of 〖 χ〗_0^2 = 646.7990, with df = 6 and p = 0.0000 < 0.01. The data supports the hypothesis.
The Testing Hypothesis 4
The interaction between the factors of Initial Knowledge and Achievement Motivation has jointly a significant effect on the bivariate Learning Result as assessed by the Analytical Rubric and Holistic Rubric.
Based on the Chi-Square Test statistic of〖 χ〗_0^2 = 116.655 with df = 2 and p = 0.0000 < 0,01. The data supports the hypothesis.