Filter Results
9412 results
Caloric sweeteners such as sugar and honey are replaced in thousands of food products by non-caloric artificial sweeteners (NAS). The present study aimed to determine the effects of chronic NAS intake on circadian sleep regulation.
Data Types:
  • Slides
  • Image
Novel amorphous silica with extremely large mesopores were prepared using the gel skeletal reinforcement method with hexamethyldisiloxane (HMDS) and acetic anhydride (AA) system as reinforcing reagents. Pore volume and pore diameter increased with increasing the amounts of HMDS added. When three times higher moles of HMDS than that of silica source TEOS were used, pore volume and pore diameter of prepared amorphous silicas reached larger than 5 cm3/g and 44 nm, respectively, even after calcination at 600 °C for 3 h. The mixed catalysts prepared with the reinforced silica, β zeolite and alumina-sol binder showed much higher conversions of n-dodecane than respective single zeolite even though these catalysts included only 26 wt% of zeolite. The activity tended to increase with increasing pore size and pore volume of the reinforced silica. When aluminum tri-sec-butoxide (ASB) was used as a gel reinforcing reagent, alumina was introduced on the silica surface. In catalytic cracking of n-dodecane the mixed catalysts using ASB reinforced silica showed higher conversion than the catalysts using HMDS, indicating that silicas reinforced by ASB formed silica–alumina in the external surface which played a role of additional acid sites.
Data Types:
  • Slides
  • Image
  • Tabular Data
The androgen receptor splice variant-7 (AR-V7) has been implicated in the development of castration-resistant prostate cancer (CRPC) and resistance to abiraterone and enzalutamide.
Data Types:
  • Slides
  • Image
  • Tabular Data
  • Document
Antibody-based immunosensors are relatively less accessible to a wide variety of unreachable targets, such as low-molecular-weight biomarkers that represent a rich untapped source of disease-specific diagnostic information. Here, we present a peptide aptamer-based electrochemical sensor technology called ‘PEP-on-DEP’ to detect less accessible target molecules, such as renin, and to improve the quality of life. Peptide-based aptamers represent a relatively smart class of affinity binders and show great promise in biosensor development. Renin is involved in the regulation of arterial blood pressure and is an emerging biomarker protein for predicting cardiovascular risk and prognosis. To our knowledge, no studies have described aptamer molecules that can be used as new potent probes for renin. Here, we describe a portable electrochemical biosensor platform based on the newly identified peptide aptamer molecules for renin. We constructed a randomized octapeptide library pool with diversified sequences and selected renin specific peptide aptamers using cDNA display technology. We identified a few peptide aptamer sequences with a KD in the µM binding affinity range for renin. Next, we grafted the selected peptide aptamers onto gold nanoparticles and detected renin in a one-step competitive assay using our originally developed DEP (Disposable Electrochemical Printed) chip and a USB powered portable potentiostat system. We successfully detected renin in as little as 300ngmL−1 using the PEP-on-DEP method. Thus, the generation and characterization of novel probes for unreachable target molecules by merging a newly identified peptide aptamer with electrochemical transduction allowed for the development of a more practical biosensor that, in principle, can be adapted to develop a portable, low-cost and mass-producible biosensor for point-of-care applications.
Data Types:
  • Slides
  • Image
Power Doppler ultrasound is used to assess joint vascularity in acute arthritis. PDUS signals have been correlated with synovial histology and bone deterioration. Little is known about the correlation between power Doppler signals and synovial white blood count. In our study, we analyzed power Doppler signals in inflammatory joint diseases including gout, calcium pyrophosphate deposition disease, rheumatoid arthritis, spondyloarthritis and others and correlated power Doppler signals with synovial white blood count and with serologic markers of inflammation.
Data Types:
  • Slides
  • Image
  • Tabular Data
Clinicians commonly interpret the findings of shoulder rotational ranges of motion using various approaches: an apparent reduction in dominant arm internal rotation (“GIRD”), a difference in total rotational range of motion—i.e. differences in the sum of internal and external rotational range (TRROM), and a combination of rotational ROM and torsional difference (“GIRD-torsion”). We have noticed that these approaches are being considered to provide equivalent estimates of shoulder rotational range. This investigation sought to document the extent of agreement of these three different approaches when classifying athletes’ “at-risk” status.
Data Types:
  • Slides
  • Tabular Data
The well recognized complex issue of compatibility between starch and gelatin was studied based on their interfacial and phase composition using Raman microscopy. Blending films with different ratios of gelatin and starch was used in this work. Raman beam focused on a 1.7 μm × 1.5 μm detection region and the micro-spectroscope scanned across the gelatin–starch interface. The ratio of areas of saccharide bonds (1173-953 cm−1) and amide I bands (1750-1550 cm−1) was used to monitor the relative distributions of the two components of the blends. The Raman spectral maps confirmed that for all the blends investigated, gelatin formed a continuous matrix in which starch inclusions were dispersed. Intermediate phases consisted of amylose interacted with gelatin, which demonstrated this was an unique and effective method to investigate starch based blend and composite materials.
Data Types:
  • Slides
  • Image
Fining treatment is an important step in winemaking but its mechanism is still not well known. To have a better understanding of the protein fining process, the influence of polysaccharides and glycerol, major compounds in wine, on proanthocyanidin precipitation was studied. A wine and a model wine obtained from its polyphenol fraction were analyzed before and after fining by a gelatin and a plant protein, a hydrolyzed gluten HG. The results showed that polysaccharides and glycerol modify quantitatively proanthocyanidin precipitation, but not much the nature of the precipitated proanthocyanidins. The nature of the remaining proanthocyanidins in the treated model wines after protein fining was not much modified neither. All proanthocyanidins were not recovered after the fining treatments when polysaccharides and glycerol were added to the model wines. These results suggested the creation of soluble complexes between proanthocyanidins, fining proteins and polysaccharides. The efficiency of the fining treatment could be thus modified by the content of the wines.
Data Types:
  • Slides
  • Image
  • Tabular Data
The sustainable, efficient use of renewable bio-based additives in the production of various materials fits well into the concept of sustainability. Here, the concept of coaggregation of mineral filler particles and starch granules for improving filler-fiber interaction in paper-based cellulosic networks is presented. Coaggregation of precipitated calcium carbonate filler particles and uncooked, unmodified corn starch granules by cationic polyacrylamide (a cationic high molecular weight polymer flocculant) in combination with bentonite (an anionic microparticle) prior to addition to cellulosic fiber slurry delivered enhanced filler bondability with cellulosic fibers. For instance, under the conditions studied, preaggregation resulted in an increase in filler bondability factor from 9.24 to 15.21 at starch dosage of 1% (on the basis of the dry weight of papermaking stock). The swelling and gelatinization of the starch granules in starch-filler preaggregates or hybrids enabled the “bridging” of the gaps in cellulosic networks, leading to structural consolidation and strength enhancement.
Data Types:
  • Slides
  • Image
Zygosaccharomyces rouxii is a yeast which can cause spoilage in the concentrated juice industries. It exhibits resistance to high sugar concentrations but genome- and proteome-wide studies on Z. rouxii in response to high sugar concentrations have been poorly investigated. Herein, by using a 2-D electrophoresis based workflow, the proteome of a wild strain of Z. rouxii under different sugar concentrations has been analyzed. Proteins were extracted, quantified, and subjected to 2-DE analysis in the pH range 4–7. Differences in growth (lag phase), protein content (13.97–19.23mg/g cell dry weight) and number of resolved spots (196–296) were found between sugar concentrations. ANOVA test showed that 168 spots were different, and 47 spots, corresponding to 40 unique gene products have been identified. These protein species are involved in carbohydrate and energy metabolism, amino acid metabolism, response to stimulus, protein transport and vesicle organization, cell morphogenesis regulation, transcription and translation, nucleotide metabolism, amino-sugar nucleotide-sugar pathways, oxidoreductases balancing, and ribosome biogenesis. The present study provides important information about how Z. rouxii acts to cope with high sugar concentration at molecular levels, which might enhance our global understanding of Z. rouxii's high sugar-tolerance trait.
Data Types:
  • Slides
  • Image
  • Tabular Data
  • File Set
6