Epoxy-tiglianes are a novel class of diterpene esters isolated from a native Australian rainforest plant, Fontainea picrosperma (Euphorbiaceae). The prototype epoxy-tigliane, EBC-46 (tigilanol tiglate), possesses potent anti-cancer properties and is currently under clinical evaluation as a treatment for cutaneous and subcutaneous tumors in humans and domesticated animals. EBC-46 also stimulates exceptional dermal wound healing in vivo upon tumor destruction, manifested as accelerated wound re-epithelialization and closure. However, little is known how epoxy-tiglianes induce such favorable epithelial wound healing outcomes.
The data presented provides supplementary information to our manuscript, demonstrating the stimulatory effects of EBC-46 and analogue, EBC-211, on the proliferative and migratory responses of human keratinocytes. Specifically, the data shows 1) The effects of both epoxy-tiglianes on the stimulation of accelerated cell cycle progression (assessed by Draq5 staining and Flow Cytometry analysis), 2) The effects of EBC-46 and EBC-211 on enhanced scratch wound repopulation (automated Time-Lapse Confocal Microscopy movies), in addition to epoxy-tigliane scratch wound movies in the absence and presence of anti-proliferative agent, mitomycin C (MC) and pan-protein kinase C (PKC) inhibitor, bisindolylmaleimide-1 (BIM-1). 3) We further provide Tables reporting the findings of Microarray analyses on the keratin (KRT); DNA synthesis/replication; cell cycle, proliferation and apoptosis; adhesion/migration; differentiation; proteinase; and cytokine/chemokine genes differentially expressed by EBC-46 and EBC-211, to facilitate enhanced keratinocytes wound healing responses. 4) Although differentially expressed genes of interest shown to be significantly different at the protein level are included in the main manuscript (by Western blotting and ImageJ densitometry), non-significant differences in Western blot protein profiles between untreated and epoxy-tigliane-treated keratinocytes are shown here.
This file contains all the coreflooding experimental data used in the current study. Each experiment structure contains the 3D porosity, permeability, and CO2 saturation maps. All 3D maps are inlet first. Part of the data is processed from datasets contained in Reynolds et al. (2018).
Contributors:Maciej Spiegel, Tadeusz Andruniów, Zbigniew Sroka
Flavonoids are known for their antiradical capacity, and this ability is strongly structure dependent. In this research the activity of flavones and flavonols in a water solvent was studied with the density functional theory methods. These included examination of flavonoids’ molecular and radical structures with natural bonding orbitals analysis, spin density analysis and frontier molecular orbitals theory. Calculations of determinants were performed: specific, for the three possible mechanisms of action – hydrogen atom transfer (HAT), electron transfer-proton transfer (ETPT) and sequential proton loss electron transfer (SPLET); and the unspecific – such as reorganization enthalpy (RE) and hydrogen abstraction enthalpy (HAE). Intramolecular hydrogen bonding, catechol moiety activity and probability of electron density swap between rings were established. Hydrogen bonding seems to be much more important than the conjugation effect, because some structures tends to form more intramolecular hydrogen bonds instead of being completely planar. The very first hydrogen abstraction mechanism in a water solvent is SPLET, and the most privileged abstraction site, indicated by HAE, can be associated with C3 hydroxyl group of flavonols and C4’ hydroxyl groups of flavones. For the catechol moiety an intramolecular reorganization to an o-benzoquinone-like structure occurs and the ETPT is favored as the second abstraction mechanism.
Models as described in the manuscript "Using machine learning with target-specific feature sets for structure-property relationship modeling of octane numbers and octane sensitivity" (Preprint submitted to Fuel).
About eighty carbonated sand paritcles were placed in three small polycarbonate pipes and then subjected to X-Ray micro-CT scanning. The .tif documents are 3D micro-CT images of these carbonate sand particles within three three polycarbonate pipes. The .tif documents can be input into 3D visualization softwares, such as ImageJ, Avizo, et al., and the 3D particle morphlogy and intra-particle pore structure of these carbonate sand particles can be visulized. Moreover, the .doc document summarizes all the carbonated sand particles in a table.
Contributors:Yasser Shafiei-alamooti, Ali Ashrafizadeh
The data is the results obtained from conjugate numerical solution of governing equations of fluid flow and heat transfer in a three stream plate-fin heat exchanger as explained in the parent paper. They consist of the ASCII text files each for one case according to table 2, and each has four sections for three fluid streams and a solid body as shown on the Fig. 3. The solution has been based on unstructured grids so each section has two parts: the nodal data and the connectivity. The files could be handled via any text editing software as well as Microsoft Excel, although it is constructed in a manner that facilitate the post-processing by TecPlot software.