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  • The contained data consist of Illumina HiSeq reads generated genomic DNA of Oryza sativa ssp. indica used for comparative coverage aspects with plant-RRBS methylome profiling by bioinformatics analyses. The inbred control line and a derived epiline LR2 of the 4th selfing were analysed using whole-genome bisulfite sequencing.
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  • Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose. 3 hours and 24 hours after transfer, seedlings were harvested and the third leaf micro-dissected for RNA extraction.
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  • CD4+ T cells were extracted from mouse and human. They were activated in vitro with CD3/28 and cultured with Il4. ATAC-seq was then performed at different time points
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  • To characterise their gene expression, wild type pancreatic pericytes were isolated based on the YFP-labeling from Nkx3.2-Cre;EYFP mice. As control, whole isolated islets were analysed.To identify Tcf7l2-depenedent genes, pancreatic pericytes were isolated from Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox transgenic mice, and compared to wild type cells.
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  • The contained data consist of Illumina HiSeq 2500 reads generated from restriction endonuclease digested genomic DNA of Oryza sativa ssp. indica used as proof of concept for plant-RRBS methylome profiling by bioinformatics analyses. Five biological repeats for an inbred control line and a derived epiline of the 4th generation where analysed using two restriction endonuclease combination (MspI-DpnII or MspI-ApekI).
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  • To assess natural variation in the ability to respond to changes in gibberellin metabolism, we examined the effect of the ectopic expression of GA20ox1 in 10 Arabidopsis thaliana accessions. RNA-seq was performed on the sixth leaf, which was micro-dissected (size < 0.25 mm2) at the beginning of the transition from cell proliferation to cell expansion.
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  • MV130 is a commercially polyvalent bacterial preparation, which has shown to reduce the the rate of respiratory infections in patients suffering from recurrent respiratory tract infections. However, the immunological mechanisms associated to this clinical benefit remain unknown. We wanted to identify immunological mechanisms in human monocytes-derived dendritic cells (hmoDCs) from 4 healthy donors treated with MV130 compared to control. Global comparative transcription by DNA technology, allows us to detect genes, pathways and clusters that are differentially expressed with the treatment (MV130) respect to control.
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  • Large scale RNA-Seq analysis was performed to investigate the transcriptomic response to osteoarthritis in cartilage and investigate potential subgroups of patients. Data were collected from intact knee cartilage (posterior lateral condyle) from at total of 60 patients with osteoarthritis (OA) following total knee replacement and 10 control non-OA patients following amputation.
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  • In the current study, we have performed a multi-factorial integrative analysis of genome-wide chromatin immunoprecipitation-sequencing (ChIP-seq) on multiple histone marks, as well as RNA-Sequencing (RNA-seq) and DNA methylation studies to generate new knowledge on the epigenetic landscapes underlying the heterogeneity of PDAC tissue grown as patient-derived tumor xenografts (PDTXs).
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  • In the current study, we have performed a multi-factorial integrative analysis of genome-wide chromatin immunoprecipitation-sequencing (ChIP-seq) on multiple histone marks, as well as RNA-Sequencing (RNA-seq) and DNA methylation studies to generate new knowledge on the epigenetic landscapes underlying the heterogeneity of PDAC tissue grown as patient-derived tumor xenografts (PDTXs).
    Data Types:
    • Text
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