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Grf10 is an uncharacterized homeodomain-containing transcription factor in Candida; this experiment was to determine potential gene targets in yeast-growth conditions.
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The RiBi project investigated the role of chromatin factor Spt6 in ribosome biogenesis. In this experiment we performed transcription profiling of Saccharomyces cerevisiae single and double deletion mutants involved in ribosome assembly. The single mutants examined in this experiment were: arx1-deletion, dbp7-deletion, mrt4-deletion, zuo1-deletion and spt6 -140 mutant. The double mutants were spt6-140 mutant plus arx1-deletion; spt6-140 mutant plus dbp7-deletion; spt6-140 mutant plus mrt4-deletion; spt6-140 mutant plus rsa1-deletion; and spt6- 140 mutant plus zuo1-deletion. The experiment was performed using a dye-swap design where a single wildtype culture was used as a common reference pool.
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Transcription profiling by array of 60 deletion mutants involved in ribosome assembly
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investigation of alternative splicing in breast tumors and sarcomas
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Comparison of the transcription profile between Enterococcus faecalis V583 strain and its mutant having a constitutive SOS response
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Mixed phenotype acute leukemia (MPAL) is a rare, poor prognosis disease. In this study we analyzed the gene expression profile of 12 primary MPALs. We addressed the question whether gene expression profile can provide useful information about the lineage of origin (myeloid or lymphoid) of the leukemic blasts.
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In this experiment, formalin-fixed paraffin-embedded male (n=15) and female (n=10) primary invasive ductal carcinoma (all ERα-positive, HER2-negative, node-negative) samples were selected and hybridized using the Almac Diagnostics Breast Cancer DSA(TM) array ADXBRCv2a520413 in order to investigate the gene expression differences between male and female breast cancer.
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Transcriptional profile of hematopoietic stem cells (HSC) isolated from multiple sclerosis patients and healthy controls.
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Non-small cell lung cancer (NSCLC, n=22) and normal adjacent control biopsies (n=18) from patients with lung cancer were obtained for Affymetrix GeneChip analysis. NSCLC samples were grouped into squamous cell carcinoma (SCC, n=11) and adenocarcinoma (AC, n=11) samples.
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Bone marrow derived macrophages from C57BL/6 or C57BL/6-Sst1S strains of mice were pretreated ± 100U/ml interferon gamma then infected for 0, 1, 2, 4, or 6h with Listeria monocytogenes. C57BL/6-Sst1S mice carry the Sst1 chromosomal locus of C3H mice. The Sst1 locus controls susceptibility to intracellular pathogens - these mice are therefore more susceptible than wild type B6.
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