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  • Abstract: Due to the high cost of bioprinters they are not feasible for proof of concept experiments or educational purposes. Furthermore, the more affordable DIY methods all disable the plastic printing capability of the original printer. Here we present an affordable bio-printing modification that is easy to install and maintains the original capabilities of the printer. The modification used mostly 3D printed parts and is based on the popular, open-source Prusa i3 3D printer. The modifications are kept as simple as possible and uses standard slicing software, allowing for installation by less experienced builders. By using disposable syringes and easily sterilizable parts, an aseptic bioprinting setup can be achieved, depending on the environment. It also allows for 2 component printing as well as UV curing.
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  • This directory contains the following datasets and supplementary materials: SUPPLEMENTARY FIGURES: The analysis method of the proposed LVRT strategy and the detailed designation process. SUPPLEMENTARY TABLES: The simulation parameters.
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  • Raw data for the article: Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems.
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  • Coherent Fourier scatterometry with non-focused, normally incident light on brain tissue samples: The measurements were performed with a collimated laser beam (with a wavelength of 633 nm and a diameter between 0.1-1 mm). The light was transmitted through histological brain sections of 30-60 um (coronal vervet brain sections and 2-3 crossing optic tracts of a human optic chiasm). The scattered light behind the sample was collected by a microscope objective and the distribution of the scattered light (Fourier transform of the image plane) was recorded by a camera. The measurements were performed for different samples (Vervet/Chiasm, brain sections s0007-493), different beam diameters (100 um or 1120 um), different numerical apertures (NA = 0.14, 0.4, 0.8), different exposure times (10 - 600 ms), and different brain regions (cc = corpus callosum, cg = cingulum, corona = corona radiata, f = fornix; different x/y-coordinates). The data set contains the Scattering Patterns (distribution of scattered light, projected onto a hemisphere behind the sample), Azimuthal Integrals (scattering pattern integrated along the azimuthal angle, plotted against the distance from the center), and the Polar Integrals (scattering pattern integrated from the center to the outer border, plotted against the azimuthal angle [0°,360°]) for all measured brain regions.
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  • 1H, 13C NMR (FID and processed spectra) and HR-MS spectroscopic data of amino-phenol chelating ligands, their derivatives with B-galactose, N,O-ligands and HR-MS spectra of Fe iron(III) complexes as bioresponsive, smart, enzyme sensitive systems
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  • Research data
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  • Androgenetic alopecia global photographs pre- and post-treatment of with 5α-reductase inhibitors (5ARI). The images compared patients before and after 5ARI treatment. Patient 1(M/55): greatly increased improvement(seven-point score: 3) after 10 months of dutasteride treatment (0.5 mg/d); patient 2(M/61): moderately increased improvement(seven-point score: 2) after 1 year of finasteride treatment (1 mg/d); patient 3(M/65): slightly increased improvement(seven-point score: 1) after 6 months of dutasteride treatment (0.5 mg/d); patient 4(M/77): slightly increased improvement(seven-point score: 1) after 9 months of finasteride treatment (1 mg/d).
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  • Human linker histone H1 is an important role player in the packaging of DNA. H1 has a tripartite structure: an evolutionarily conserved central globular domain that adopts a winged-helix fold, flanked by the highly variable and intrinsically unstructured N- and C-terminal domains. This dataset consists of raw 2D and 3D BEST-TROSY NMR data recorded on Bruker Avance III HD spectrometers, operating at 700 or 950 MHz 1H frequency, and equipped with cryogenically cooled triple-resonance (HCN) probes and pulsed z-field gradients at 5'C (278 K). Data were recorded for NGH1x [residues 1 – 120 of human H1x; UniProtKB: Q92522(H1X_HUMAN) consisting of the N-terminal and globular domains] in 20 mM sodium phosphate (‘low salt’) or 20 mM sodium phosphate + 1 M sodium perchlorate (‘high salt’). Data can be analyzed using NMR spectra analysis software such as Sparky, CCPNMR, etc. A description of the data folders is given in the file "NMR Description of data folders.pdf".
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