Mendeley Data Showcase

This plasmid was used for genetic introduction of a second copy of the FtsZ gene fused with mCherry to JCBI-syn3B for fluorescent imaging of cell division. Gibson assembly was used to construct the plasmid. Syn1.0 (GeneBank accession number CP002027) served as the template to amplify fragment 1 using primers 5’-GAATTCGCCAGAACCAGCAGCGGAGCCAGCGGATCCTTTTAAAAATGTCGGAAAGTCATC-3’ and 5’-AGCAAAGTGGGTGATAAATAAATGACAAACGAATTTAAACAAATAGC-3’, and fragment 2 using primers 5’-CGTTTGTCATTTATTTATCACCCACTTTG-3’ and 5’-ATTTGAACGTTGCGAAGCAACAGAAGCATAATAACAATTATTAAT-3’. The vector backbone was amplified using primers 5’-AAGGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATTCATGGTATCAAAAGGAGAAGAAGATAATATG-3’ and 5’-TAATTGTTATTATGCTTCTGTTGCTTCGCAACGTTCAAATC-3’, with the ptxB-FLAG plasmid (not published) as the template. PrimeSTAR Max DNA polymerase (Takara, #R045A) was used to amplify the fragments. PCR was performed with an initial denaturation at 98 °C for 3 minutes, followed by 30 cycles of 98 °C for 10 seconds, 55 °C for 10 seconds, and 72 °C for 1 minute, with a final extension at 72 °C for 5 minutes. Primers 5’-TCCTCCAGCTCCTAATCCTT-3’ and 5’-TGTTTGTCGGTGAACGCTCT-3’ were used for colony PCR to identify positive clones. The final plasmid was confirmed by sequencing.

The tropical deciduous forests of the Dang district in South Gujarat represent one of the most biodiverse regions in Western India. This study was conducted to document the avifaunal diversity and analyze how tree species composition, phenology, and structural complexity influence bird community dynamics. The primary objective was to quantify species richness and abundance while mapping the ecological niches occupied by different bird guilds in relation to specific tree species. Data were collected using point counts and opportunistic observations. For every bird sighting, the following parameters were recorded: species identity (Order, Family, Common Name), number of individuals, activity (e.g., feeding, calling, perching), and detailed substrate information (tree species name, GBH, tree height, and phenology). Canopy positioning was further categorized into vertical (Lower, Middle, Upper) and horizontal (Inner, Outer) strata to understand microhabitat preferences. A total of 1,003 individuals were recorded, representing 68 bird species belonging to 39 families and 16 orders. The study revealed a highly diverse community with a Shannon-Wiener Diversity Index (H') of 3.60, indicating a well-distributed and ecologically stable population. The Order Passeriformes was the most dominant, with the family Leiothrichidae (e.g., Jungle Babblers) showing the highest abundance. Other significant families included Columbidae (Pigeons and Doves) and Estrildidae (Munias). The analysis of feeding guilds showed a predominance of Insectivorous and Granivorous birds. A critical finding was the strong association between bird presence and specific tree species. Acacia nilotica, Tectona grandis, and Ficus species emerged as vital substrates for perching and feeding activities. Phenological data indicated that trees in the Fruiting and Flowering stages attracted significantly higher bird counts, particularly nectarivorous species like the Purple Sunbird (Cinnyris asiaticus) and frugivores. Birds utilized different canopy layers based on their feeding habits. Insectivores were predominantly found in the middle and upper canopies, while granivores were often associated with the lower canopy or ground level. The "Inner" canopy was frequently used for perching and nesting, providing protection, whereas the "Outer" canopy was the primary site for active feeding and calling. The high species richness and diversity index underscore the conservation importance of the Waghai forest region. The significant correlation between tree phenology and bird activity suggests that maintaining a diverse tree species composition is essential for sustaining the avifaunal community. These findings provide a baseline for future conservation efforts and habitat management in the Dang district, highlighting the need to protect old-growth trees and diverse floral species to maintain ecological balance.

Raw data of the published paper - https://doi.org/10.1021/acscatal.5c05963 Cite this: ACS Catal. 2025, 15, 23, 20036–20048

Source File

Replication package for "Insufficient or Excessive Investment Under Sovereign Default Risk", Conditionally accepted at the Journal of International Economics Abstract: Private agents do not internalize the impact of their investment decisions on the sovereign’s bond prices and default risk. Therefore, a standard externality argument implies that investment is insufficient and that a subsidy can improve welfare, if financed by non-distortionary means. We contrast this logic with a countervailing force. When the sovereign is impatient relative to households, plausibly due to political economy factors, it finds laissez-faire capital accumulation excessive and might prefer instead to tax it. We embed both mechanisms in a sovereign default model with decentralized capital investment, long-term public debt, and stochastic trend growth, calibrated to salient features of the Spanish economy. We find that the impatience channel dominates quantitatively, to such an extent that laissez-faire is preferable to the government’s ideal fiscal policy, based on households’ welfare.

Содержание включает наборы данных и код, относящиеся к теме исследования.

The dataset was constructed using several Russian data sources. Bond- and issuer-level information was obtained from the Cbonds database and SPARK. Trading data were collected from the Moscow Exchange. Macroeconomic and financial indicators were taken from the Bank of Russia and Rosstat.

Echocardiography parameters of non-DOX Ly6G depleted mice. LV, left ventricle; LA, left atrium; IVRT, isovolumic relaxation time; IVCT, isovolumic contraction time; LVEDD, LV end diastolic dimension; and LVESD, LV end systolic dimension.

Resistance gene calls from CARD, ResFinder and BacMet were integrated into a single curated presence/absence matrix, removing duplicate detections across databases so that each gene was represented once per genome

(A) Representative flow cytometric plots of CD11b+Gr1+ cells isolated from heart of rIgG- or αLy6G-treated mice. (B) Percentage of body weight change. (C-D) MW/TL (C), and percentage change of MW/TL (D) of rIgG- or αLy6G-treated mice (n=5). (E-F) HW/TL (E) and percentage change of HW/TL (F) of rIgG- or αLy6G-treated mice (n=5). (G) Representative echocardiographic images (M-mode) showing left ventricular internal diameter end diastole and end systole. Cardiac functions including the percentage of ejection fraction (H-I), cardiac output (J-K), and stroke volume (L-M) of rIgG- or αLy6G-treated mice (n=5). (N) Representative Picrosirius Red staining of cardiac tissue from control or long-term (D4w+4w) DOX treated mice (n=4-5). (O) Quantification of fibrotic area normalized to cardiomyocyte area of control or DOX treated mice (n=4-5).