Dataset on Antimalaria Drug-drug Interactions in mice
Description
This data was obtained from the study aimed at determining changes in parasitaemia and selected liver and kidney function parameters in NK 65 Plasmodium berghei passaged mice treated with oral coadministration of artemether lumefantrine with rifampicin, ciprofloxacin, erythromycin, caffeinated paracetamol, tea extract grapefruit juice and beer. The data comprises of percentage parasitaemia on days 3, 6 and 9 determined by microscopy using thin blood smears taken from the tail of the mice on day 3 before treatment commenced and on days 6 and 9. It also contains the alanine amino transferase (ALT) aspartate amino transferase (AST) activities, serum total and direct bilirubin, Urea and creatinine concentrations collected on day 9 and determined from optical density values.
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Using Rane’s test, 51 albino mice were randomized into 17 groups of 3 mice each. Groups A and B were the uninfected and infected control groups treated with water while Groups C through to Q were the infected groups treated with artemether lumefantrine administered with ciprofloxacin, erythromycin, rifampicin, caffeinated paracetamol, tea extract, ethanol and grapefruit juice. Using Rane’s test, 51 albino mice were randomized into 17 groups of 3 mice each according to the following design; GROUPS TREATMENT GROUP A Uninfected mice given water GROUP B NK65 Plasmodium berghei passaged mice given water GROUP C NK65 Plasmodium berghei passaged mice treated with Artemether lumefantrine GROUP D NK65 Plasmodium berghei passaged mice treated with Ciprofloxacin GROUP E NK65 Plasmodium berghei passaged mice treated with AL co-administered with Ciprofloxacin GROUP F NK65 Plasmodium berghei passaged mice treated with AL administered with Ciprofloxacin at an interval of 2 hours GROUP G NK65 Plasmodium berghei passaged mice treated with Rifampicin GROUP H NK65 Plasmodium berghei passaged mice treated with AL co-administered with Rifampicin GROUP I NK65 Plasmodium berghei passaged mice treated with AL administered with Rifampicin at an interval of 2 hours GROUP J NK65 Plasmodium berghei passaged mice treated with Erythromycin GROUP K NK65 Plasmodium berghei passaged mice treated with AL co-administered with Erythromycin GROUP L NK65 Plasmodium berghei passaged mice treated with AL administered with Erythromycin at an interval of 2 hours GROUP M NK65 Plasmodium berghei passaged mice treated with AL co-administered with caffeinated paracetamol GROUP N NK65 Plasmodium berghei passaged mice treated with AL administered with caffeinated paracetamol at an interval of 2 hours GROUP O NK65 Plasmodium berghei passaged mice treated with AL co-administered with grapefruit juice GROUP P NK65 Plasmodium berghei passaged mice treated with AL co-administered with aqueous tea extract GROUP Q NK65 Plasmodium berghei passaged mice treated with AL co-administered with alcoholic beverage The treatment commenced on day 3 post inoculation through to day 5 using steel canula. Antimalarial activity was determined by thin blood smears taken from the tail on day 3 before treatment commenced and on days 6 and 9. Determination of Biochemical Parameters Randox assay kits were used for determination of the biochemical markers of tissue toxicity and used according to manufacturer’s instructions. Liver function enzymes alanine amino transferase (ALT) and Aspartate Amino Transferase (AST) activities were determined by the method of Reitman and Frankel (1957) while bilirubin concentration was determined by the method of Sherlock (1951). Serum creatine, was determined by the method of Bartels and Bohmer (1972) while urea concentration was determined by urease-Berthelot method of Wheatherburn (1967)