Somatic hypermutation in Male and Female mice
Somatic hypermuation sequences associate with the manuscript "Greater Breadth of Vaccine-Induced Immunity in Females than Males Is Mediated by Increased Antibody Diversity in Germinal Center B Cells" currently in press at mBio. Data include JH4-intron sequencing from male and female mice after vaccination and boosting with Flu antigen. Sequences were trimmed to remove primer sequence and contain a small fragment of the FW3 of the VH1 family in mice and end ~500 bp downstream of JH4. Sequences were isolated from germinal center B cells (CD19+CD38-GL7+), amplified by nested PCR, and sanger sequenced.