Unprocessed and up-cropped Western blot images [Pharmacol. Res. (2021) 166:105474]

Published: 16-03-2021| Version 2 | DOI: 10.17632/26n8thh5g9.2
Chun Hei Antonio Cheung


In our previous study [Cheng et al., Pharmacological Research (2021) 166:105474] , we demonstrated that ROS scavenging by the use of Tiron partially attenuated the DNA damaging effects of BIRC5 down-regulation in MCF7 and MDA-MB-231 cells, as shown by the results of the Western blot analysis (Fig. 3E). Despite the ACTA1 blots (i.e. the blot images) used for the "MCF7 and MDA-MB-231 panels" are completely different and without manipulation; however, these blots "on certain degree" look a bit similar to each other. In order to avoid creating confusion to the reader, we here provide the Raw (unprocessed and up-cropped, and with a series of different exposure time) images in order to support the authenticity of the images published (Fig. 3E) in the article. On the images, the first 4 lanes belong to MCF7 and the next 4 lanes belong to MDA-MB-231. Out of these "4 lanes", only the first 3 lanes were used (i.e. Lane 1, 2, 3 and 5, 6, 7) for the figure.