Characterization and Antimicrobial Efficacy of Silver Nanoparticles Synthesized from parts of Endemic Species Terminalia pallida Brandis
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The present data submitted here is related to my research work. In this paper, I've mentioned the work related to the synthesis of silver nanoparticles, their characterization, and their three applications. UV- VIS:- (UV-VIS-NIR spectrophotometer, Jasco Make, Japan Model: V-670) - at VIT, Vellore; FTIR Modal: IR Affinity-1, (Shimadzu, Mode of Analysis: ATR Mode, Japan) at VIT, Vellore; XRD:- Bruker D-8 advance, Germany at VIT, Vellore; AFM:- (Bruker USA), ICON (Dimension) Scan Asyst at IISc Bangalore; HRSEM:- Hitachi S-3400 N SEM at IISc Bangalore; FeSEM:- (SUPRA 55) Carl Zeiss, Germany at Sathyabama University, Chennai; HRTEM:- FEI-TECNAI G2-20 TWIN (200kV) at VIT, Vellore. Applications I. Against Gram-Negative strains (-Ve): Escherichia coli and Staphylococcus aureus; Gram-Positive Strains (+Ve): Klebsiella pneumoniae and Bacillus subtilis. Description About this Research work Terminalia pallida is a semi-evergreen endemic tree. The leaf, bark, and fruits are known for their medicinal and therapeutic values. These parts have extraordinary medicinal characteristics in curing various diseases and most of the tribal people are still using the extracts of these plant parts in their daily life and curing various ailments. A lot of work had been carried out on the biosynthesis of metal and mineral nanoparticles using numerous plants. Green synthesis of nanoparticles using traditional endemic medicinal plants is scanty. So far no work has been carried out on the production of silver nanoparticles by using leaf, bark, and fruits of T. pallida. In this connection, the research work aimed to synthesize nanoparticles from plant parts individually. These biosynthesized silver nanoparticles are exhibiting antimicrobial activity which showed the efficacy of check microbial growth, along with anti-scavenging and anti-inflammatory activities. Depending on the size of SNPs they were used for different applications such as antioxidant, anti-inflammatory, and antimicrobial activity and the results showed that the SNPs have efficacy in controlling different diseases and disorders successfully when compared to the other raw forms at the nano-level, thus proving their efficiency in controlling microbes and other activities. The plant parts of Terminaliapallida can be further explored for their various uses concerning SNPs and their applications in neutraceuticals and pharmaceutical industries in the future.
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This Research paper has huge data which includes the collection of plant materials to Application results. The step-by-step process includes the 1. Extraction of plant extracts (3 parts) 2. Preparation of 1mM AgNO3 solution 3. Synthesis of Silver Nanoparticles (SNPs) 4. Characterization of SNPs by using sophisticated instruments includes UV-Vis, FTIR, AFM, XRD, HRSEM, FESEM, and HRTEM. With these instruments, and by using Origin software, it is easy to find the formation of SNPs to their position, structures, size (5 - 50nm), and other things such as agglomeration, etc. 5. The SNPs are subjected to application part antimicrobial activity with disc diffusion method, against two gram-negative and two gram-positive bacterial strains showing the impact of SNPs (zones) in controlling these microbes. This confirms that SNPs can be used as a solution against microbes. For this work, the material used is Hi-media agar, 90 mm Petri dishes, Incubator, Laminar airflow, an autoclave, cotton buds for swabbing, conical flasks, test tubes, absorbent, and non-absorbent cotton, polyethylene covers, porcelain plates, discs from filter paper, etc.