Long non-coding RNA MALAT1 promotes the proliferation and metastasis of lung cancer by regulating FUT4 and α1,3-fucosylated glycans mediated via the PI3K/Akt signaling pathway
Description
Long non-coding RNAs (lncRNAs) including metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) are identified as activators in lung cancer with elusive mechanisms. Fucosyltransferase IV (FUT4) is the key enzyme that catalyzes the biosynthesis of α1,3-linkage fucosylated glycans carried by glycoproteins on the cell surface, i.e., tumor-associated sugar antigen Lewis Y (LeY). An abnormal increase in the level of FUT4 and LeY is observed in many cancer types and is correlated with cell proliferation and metastasis. Our study aims to investigate the role of MALAT1 in NSCLC and its potential interactions with FUT4 in promoting the progression, proliferation, and metastasis of NSCLC cell lines.
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MALAT1 expression was assessed using quantitative PCR (qPCR) while FUT4 expression was measured using qPCR, enzyme-linked immunosorbent assay (ELISA), and Western blotting in both serum and tissue samples. To confirm the regulatory role of MALAT1 in FUT4 expression, Western blotting, qPCR, and immunofluorescence techniques were employed in A549 and H1299 cell lines. LeY in the epidermal growth factor receptor was detected using Immunoprecipitation.