Research Data from: In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

Published: 11 April 2023| Version 1 | DOI: 10.17632/28cnrjw3vm.1
Marta Trzaskowska, Vladyslav Vivcharenko, Paulina Kazimierczak, Agata Wolczyk, Agata Przekora


The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(-)-limonene, sabinene, carvacrol, thymol, alpha-pinene, beta-pinene, and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs). This is a dataset related to the recently published article (Biomedicines 2023, 11, 1095. Each GraphPad Prism file (.pzf) contains the results of LDH total proliferation assay for individual essential oil compound (file name indicates specific compound). GraphPad Prism 8.1.2 Software was used to generate these files. In “Data Table” you should select the name of the compound to see the results obtained for 3 repetitions of the test. The results are expressed as a “cell number x 104”. First row shows results for “24 hours” time interval and second row shows results for “72 hours” time interval. Group A always shows control cells, whereas Group B and Group C show different concentration of tested essential oils-derived compound. Each Microsoft Excel file (.csv) contains the results of either ARS mineralization test or ELISA for osteogenic markers (file name indicates the type of osteogenic marker or the cell type used for ARS mineralization test). The results of ARS are expressed as OD values. ELISA results are shown as concentration of specific osteogenic marker. Column B indicates control cells, columns C-R show results for different concentrations of tested essential oils-derived compounds. Abbreviations: DT – doubling time OC – osteocalcin MC3T3-E1 – mouse preosteoblasts ADSCs – adipose tissue-derived mesenchymal stem cells ARS – Alizarin Red S staining



Uniwersytet Medyczny w Lublinie


Pharmacy, Pharmacognosy, Bone Development, Adult Health


Narodowe Centrum Nauki


Ministry of Education and Science in Poland