Myotube Baf60c controls muscle regeneration via Dkk3-mediated paracrine signaling in mice and humans
Description
In this study, we reveal that Baf60c in mature skeletal myocytes modulates muscle stem cell function and regeneration through Dkk3-mediated paracrine signaling. Using mouse models with muscle specific knockout or transgenic expression of Baf60c, we uncovered an unexpected role of Baf60c in mature skeletal myocytes in governing muscle stem cell reparative function and regeneration capacity through production and secretion of myokines. Moreover, we identity Dkk3 as the downstream target of Baf60c in mature myocytes, contributing to deterioration of muscle regeneration capacity and progressive decline in functional muscle mass in obesity and type 2 diabetes. These datasets contain processed RNA-Seq, ATAC-Seq and Microarray data in skeletal muscles from Baf60c f/f mice and BcMKO mice (generated by crossing Baf60c f/f mice with MLC Cre mice). Details in the sample collection and data analysis pipeline can be found in the Method section of the manuscript. RNA-Seq: BcMKO_RNAseq.csv: RNA-Seq data from the tibialis anterior muscles of Baf60c f/f and Baf60c f/f MLC Cre mice following injection with either PBS or CTX for 3 days (referred as CTR_PBS, CTR_CTX, CKO_PBS and CKO_CTX, respectively), in 3 biological replicates. ATAC-Seq: Baf60c_f/f.bw, Baf60c_f/f_MLC_Cre.bw: BigWig files of ATAC-Seq from the quadriceps muscles of Baf60c f/f and Baf60c f/f MLC Cre mice, in 6 biological replicates. Microarray: BcMKO_Microarray.csv: Microarray data from the quadriceps muscles of Baf60c f/f and Baf60c f/f MLC Cre mice, in 3 biological replicates.