Research hypothesis: Administration of aqueous extract of Agnus castus leaves to pcos-induced female Wistar rats is beneficial What data shows: the current data shows the administrations of Agnus castus at all doses administered led to an increase in the activities of Alkaline Phosphatase, catalase, and superoxide dismutase from 1357.32 nM/min/mg, 0.020 U/mg protein and 361.11 U/mg to 2230.64 nM/min/mg, 0.10 U/mg protein, and 583.33 U/mg protein respectively. Also, glucose (175 mg/100mg to 691.67 mg/100mg) and cholesterol levels (173.77 mg/dl to 182.81 mg/dl) increased when compared with the letrozole administered rats. There was a slight depletion in the levels of uterine protein (from 1.23mg/g to 1.13mg/g). Serum levels of testosterone increased in letrozole administered rats but decreased in animals that received Agnus castus extracts. Follicle stimulating hormone and estradiol level increased in animals administered with Agnus castus compared with letrozole administered animals. Notable findings: - Elevated testosterone level was reduced in Agnus castus treated rats - Lipid peroxidation levels reduced considerably in Agnus castus treated rats - Reduced level of antioxidant enzymes was augmented after Agnus castus treatment How the data can be interpreted: Although Agnus castus aqueous leaves extract seemed to show some beneficial effects on letrozole-administered pcos animals, the changes observed were not significant.
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The following methods and assay procedures were used: Reduced glutathione (Jollow et al. 1974), Catalase (Ashru and Sinha 1971), Malondialdehyde (Varshney and Kale 1990), Superoxide dismutase (Misra and Fridovich 1972), Total protein (Gornall et al. 1949), Alkaline phosphase (Wright and Plummer 1972) and Cholesterol (Roeschlau et al. 1974)