Rescue of α-synuclein aggregation in Parkinson’s patient neurons by synergistic enhancement of ER proteostasis and protein trafficking

Published: 8 November 2021| Version 1 | DOI: 10.17632/29t6p4wrs7.1
Contributor:
Joseph Mazzulli

Description

Raw western blot data for main and supplemental figures to accompany Stojkovska et al, Neuron, published online Nov 17th, 2021 (http://doi.org/10.1016/j.neuron.2021.10.032). Please see the publication for details. Non-specific or irrelevant bands that could not be validated to react with the protein of interest are indicated by an asterisk. Red boxes indicate the area used for the main figures in the Neuron publication. Cropped out lanes were either due to loading of samples that were not relevant for that particular experiment, or were misloaded on the gel and therefore not representative data. We use a multi-channel western blot detection system that permits simultaneous detection of rabbit and mouse secondary antibodies on the same blot. The channels are indicated in the figure as either 700 or 800, corresponding to excitation wavelength in nanometers. Many blots were sequentially probed by multiple antibodies for efficiency purposes, and some images therefore contain residual signals from previous antibody probes. Coomassie images refer to the gel that was used to match with the transferred blots.

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Institutions

Northwestern University

Categories

Western Blot

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