Dataset for "Multiplexed Digital Detection of B-ALL Leukemia Fusion Transcripts using the NanoString nCounter System" Publication
This dataset contains the validation data and analysis result for designing and developing a barcode-based nanoString nCounter technology for multiplexed detection of B-ALL leukemia fusion transcripts. Chromosomal rearrangements resulting in fusion transcripts have been reported in precursor B-cell acute lymphoblastic leukemia (B-ALL). Identification of fusion events is critical in the diagnosis and treatment of B-ALL. In this study, we used nanoString technology to design, validate and evaluate a multiplex panel for detection of B-ALL fusion- transcripts. Fifty-one B-ALL fusion transcripts reported in children in the literature were included in the design of the nanoString panel. Twenty-six fusion transcripts were validated using 64 positive control samples and 74 negative control samples with 100% sensitivity and 99% in comparison to RT-PCR. Our results support a potential role of nanoString as a robust and cost-effective technique that could be used in the detection of fusion transcripts and implemented in diagnostic algorithm of B-ALL. Please refer to more detailed description under each file inside the folders.
Steps to reproduce
RNAs were extracted from patient samples and ran on nanoString nCounter system to produce raw data, which was pre-processed using nanoString nSolver and analyzed using RStudio to produce biographical output for visualization.