Data presented in Brinkmann et al., 2021, Aquat. Toxicol.

Published: 25 January 2021| Version 1 | DOI: 10.17632/2d4hcr5cb5.1
Bregje Brinkmann


These files include all data presented in: Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 16SrRNA_(...) fasta and fastq formatted 16SrRNA sequences (Fig. 3c/ Table S4) with in the description line: * Colony numbers corresponding to the numbers included in Table S4 * The applied sequencing primer (27F) * NP exposure: nTiO2 (5 mg/L) or Control (no NPs) * A code referring to the parental zebrafish family that was crossed to obtain the larvae (A7, A8, or A9) 2photon_(...) * Two-photon scans of Fig. 2 * ROI sets of Fig. 2b/e (zipped) * Wavelength and focus information (.dat files) CLSM_(...) * CLSM scans of Fig. 3b (zipped) * ROI (zipped) * Metadata, where scanID corresponds to scan and ROI numbers of the zipped data CFUcounts_(...) CFU count data (Fig. 4/ Fig. S7): * Compound: Exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Date: Date of CFU isolation * DilutionFactor: Counted dilution * CFU_Larvae: CFU count per egg or larva DLS_(...) DLS measurements for nTiO2 (Table 2): * Time: Incubation time (h) * Concentration: Nominal concentration (mg/L) * ZP: Zeta potential (mV) * d_size: Z-averag size (nm) DoseResponses_(...) Mortality data of zebrafish larvae exposed to nTiO2 from 3-5 dpf (Fig. S6): * Concentration: Nominal exposure concentration (mg/L) * Day: Exposure time (days). * Mortality: Percentage of larvae that had died * Date: The date at which the mortality was scored EcoPlate_(...) EcoPlate measurement data (Fig. 5/ S3/ S8/ S9): * Compound: Zebrafish egg exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or Control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Time: Incubation time (h) * Date: Date of the measurement * EcoPlate: Number of the EcoPlate * Measurement: Measurement number (3 measurements per plate) * 32 substrates (final 32 columns): OD at 590 nm measured for the given substrate PIXE_(...) PIXE measurements (Fig. 1/ Table S2): * ExposureConcentration: Nominal concentration of nTiO2 (mg/L) * Replicate: Replicate number * EggCount: Number of eggs that was analyzed * Date: Date at which the sample was taken * mL_EggWater: Volume of egg water (mL) that was analyzed along with the eggs * Element: Measured element * Total_ppm: Elemental concentration (ppm) * LOD_ppm: LOD (ppm) * ng_Element_per_egg: Elemental concentration (ng/egg) * LOD_ng_per_egg: LOD (ng/egg) TiO2Sedimentation_(...) ICP-OES measurements of Ti for sedimentation analyses (Fig. S4): * Time: Incubation time (h) * ExposureTiO2: Nominal concentration of nTiO2 (mg/L) * MediaTi: Measured concentration of Ti (mg/L) * MediaTiO2: Measured concentration of TiO2 (mg/L) UVspectra_(...) UV-light spectra (Fig. S1): * wavelength: Measured wavelength (nm) * irradiance: Measured irradiance (uW/cm2/nm) * spectrum: Number of the acquired spectrum


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For details of these experiments, we refer to the linked publication in Aquatic Toxicology: Brinkmann BW, Beijk WF, Vlieg RC., Van Noort SJT, Mejia J, Colaux JL, Lucas S, Lamers G, Peijnenburg WJGM, and Vijver MG, 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. All text files are tab-delimited. Abbreviations: * CFU: colony-forming units * CLSM: confocal laser scanning microscopy * DLS: dynamic light scattering * dpf: days post-fertilization * ICP-OES: inductively coupled plasma optical emission spectrometry * LOD: limit of detection * nAg: silver nanoparticles (NM-300 K) * NPs: nanoparticles * nTiO2: titanium dioxide nanoparticles (NM-105) * OD: optical density * PIXE: particle-induced X-ray emission * ROI: region of interest This work was supported by the project PATROLS of European Union’s Horizon 2020 research and innovation programme under Grant number 760813.


Danio Rerio, Aquatic Toxicology, Nanotoxicology, Ecotoxicity