Supplemental Datasets for "SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stress"
Description
Below is a brief description of each supplementary dataset. For more details, please see the Materials and Methods section of the manuscript here: https://doi.org/10.1101/2023.06.05.543769. Dataset S1: LC-MS/MS based protein quantification of the “Control IP”, 5 distinct “single RBP IPs” and “Pooled IP targeting 10 RBPs” (see STAR Methods for details), related to Figure S2. Dataset S2: LC-MS/MS based protein quantification of the “HEK293T total lysate” and the supernatants after the “Control IP”, 5 distinct “single RBP IPs” and “Pooled IP targeting 10 RBPs” (see STAR Methods for details), related to Figure S2. Dataset S3: LC-MS/MS based protein quantification of the “Control IP” and “Pooled IP targeting 39 RBPs” (see STAR Methods for details), related to Figure S1. Dataset S4: LC-MS/MS based protein quantification of the HEK293T total lysates generated either from “Control (Solvent)”- or “Torin1”-treated cells (see STAR Methods for details), related to Figure 7 and Table S6. Dataset S5: LC-MS/MS based protein quantification of the 4EBP1 IP from HEK293T lysates generated either from “Control (Solvent)”- or “Torin1”-treated cells (see STAR Methods for details), related to Figure S7. Dataset S6: LC-MS/MS based protein quantification of the IgG IP and V5 IP from HEK293T lysates (see STAR Methods for details), related to Figure S2.
Files
Steps to reproduce
Please refer to the method section in the paper which can be found of the manuscript here: https://doi.org/10.1101/2023.06.05.543769