8-Oxoguanine DNA glycosylase 1 reads 8-oxoguanine in respiratory syncytial virus RNA to enhance viral amplification capacity
Description
Recent insights into oxidative stress induced guanine oxidation (8-oxoGua) have led to a paradigm shift, redefining it from merely a damaged base to a carrier of non-encoded regulatory information. Although the biological effects of 8-oxoGua can be mediated by 8-oxoguanine DNA glycosylase 1 (OGG1), the implications of OGG1 reading 8-oxoGua in viral RNA remain somewhat enigmatic. Here we report that 8-oxoGua within the respiratory syncytial virus RNA is an acquired phenotype during virus replication in the host cell. Through mass spectrometry-based proteomic analysis, we have found that OGG1 is hijacked by the viral ribonucleoprotein complexes. Finally, we demonstrate that impeding OGG1's ability to interpret 8-oxoGua sites attenuates viral replication. Overall, our results contribute to a more comprehensive understanding of RNA epigenetics and suggest that targeting the reading function of OGG1 in the viral lifecycle presents potential as a therapeutic intervention in clinical applications.