Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro

Published: 23 January 2020| Version 1 | DOI: 10.17632/2kw7tgph94.1
Contributors:
Changho Chun,
Alec Smith,
Deok-Ho Kim

Description

The immunostained image analysis of neurons created, NTA analysis of obtained EVs, image-based demonstration of EV-uptake by neurons, FACS analysis to quantify apoptotic behavior of cultures of interest, beta-galactosidase result to explain cell senescence status in each group, patch clamp experiment result to demonstrate the electrophusiological effect of astroglial EV treatment.

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Institutions

University of Washington

Categories

Confocal Microscopy, Flow Cytometry, Patch Clamp Technique

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