Data : the effects of cellulase treatments on pink guava by-products
Description
The solid waste generated from pink guava (Psidium guajava L.) puree processing is fiber-rich and it has limited nutrient digestibility and bioavailability. We investigated the effect of cellulase on structure-fermentability of the by-products, namely Refiner, Siever and Decanter. We removed most of the soluble sugar from the by-products, then estimated the hydrolysis of the different guava by-products by comparing the reducing sugar content in the effluent after treatment (1). We tested the sugar content in the distilled water used to wash the guava by-products by using the 3,5 dinitrosalicylic acid assay (2). We applied cellulase treatment on the by-products (Refiner, Siever and Decanter) and separated them into ethanolic extract (EEC) and alcohol insoluble fiber (AIF). We determined total soluble carbohydrate content and the molecular weight distribution of the soluble carbohydrates and prebiotic activity of the EEC. We also quantified the bile acid-binding capacity, and structural properties of the AIF. Cellulase treatment has led to the dissolution of the insoluble fiber (3). Siever and decanter showed a increase in total soluble carbohydrate content after cellulase treatment (4). Molecular weight distribution profile of soluble carbohydrates in the EEC showed a shift from high to low (5). The EEC of all cellulase-treated guava by-products exhibited higher prebiotic activity scores compared to those of the untreated samples (6). Cellulase treatments also affected crystallinity index as observed through X-ray crystallography analysis (7). Changes in the structure lattice were manifested as an improved capacity in binding bile acids (8). 1. Hydrolysis extent of guava by-products 2. Reducing sugar content in 1st washing and 2nd washing of the guava by-products. 3. Alcohol Insoluble Fiber (AIF) Yield 4. Total soluble carbohydrate content in the ethanolic extract (EEC) 5. Molecular weight distribution of soluble carbohydrates in different guava by-products. 6. Prebiotic activity score (raw data) 7. X-ray diffraction data of untreated and cellulase-treated guava by-products. 8. Bile-acid binding capacity
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Steps to reproduce
1. Steps to reproduce are stated in the uploaded file 2. For X-ray crystallography Data The CrI was calculated based on: Crystallinity index (CrI) (%) = where I002 = Maximum intensity of peak at 2θ Iam = Minimum intensity of peak at 2θ