Dataset of Cell Growth Regulator with EF-Hand Domain 1 (Cgref1) - a hepatokine study
Description
This dataset contains the original sources of the data included in a manuscript of our group that will be/has been published. The labels of each file are corresponding to the figures described on the manuscript or journal. Individuals may refer to the journal for specific experimental details. Fig 1C. Liver lysates of CREB-H KO mice injected with AAV-CREB-H-ΔTC or AAV-eGFP. Fig 1E. Huh7 cell culture medium and/or lysates after transfection of CREB-H-FL, CREB-H-ΔTC and vector control. Fig 2B. Immunoprecipitation of Cgref1 protein from mouse sera. Fig 2D. Cgref1 expression in wild type mouse tissues after 3 months of ND or HFD consumption Fig 2F. Cell culture media and lysates of Hepa1-6 and Huh7 transfected with Cgref1-V5 or CGREF1-V5. IP was performed on cell culture media to capture V5-tagged proteins. Fig 4C. Akt S473 phosphorylation in eWAT, sWAT and livers of WT and Cgref1-/- mice. Fig 4D. Akt S473 phosphorylation in eWAT of Cgref1-/- mice intraperitoneally injected with recombinant Cgref1 protein or PBS. Fig 4F. Analysis of insulin signaling pathway components in eWAT of WT and Cgref1-/- mice. Fig 4G. Glut4 expression in eWAT of WT and Cgref1-/- mice. Fig 4H. Glut4 expression in 3T3-L1 preadipocytes after incubation with Cgref1 protein. Fig 5G. Expression of Acc1 and Scd1 proteins in the liver samples of WT and Cgref1-/- mice.