GCA-Biological Fe(II) and As(III) Oxidation Immobilizes Arsenic in Micro-oxic Environments
Description
the objectives of this study were as follows: (i) to discern the effect of arsenic speciation on the abundance and distribution of microaerophilic FeOB, (ii) to investigate microaerophilic microbial Fe(II) oxidation processes and the products of arsenic immobilization, and (iii) to explore the mechanism of As(III) and As(V) immobilization during microaerophilic microbial Fe(II) oxidation in micro-oxic paddy soils. Soil samples from a paddy field in southern China were used as the inoculum to enrich microaerophilic FeOB using gradient tubes with FeS as the Fe(II) source. The cell growth, Fe(II) oxidation, and chemical speciation of arsenic were determined, and the biogenic minerals were characterized using Mössbauer spectroscopy and X-ray photoelectron spectroscopy (XPS). The changes in the composition and abundance of the microbial community caused by different treatments were profiled over time using 16S rRNA gene-based high-throughput sequencing. Furthermore, the microbial As(III) oxidase genes associated with the different treatments were examined quantitatively in real-time (qPCR) to evaluate the microbial As(III) oxidation.The raw data.xlsx contains the all raw data from Figure 1 to Figure 6 in manuscript. The genomic datasets are submitted to NCBI. You can use BioProject ID and accession number to download the data if you want (the ID and number are provided in manuscript and Raw data.xlsx).