Live Imaging of SARS-CoV-2 Infection in Mice Reveals that Neutralizing Antibodies Require Fc Function for Optimal Efficacy by Ullah et al_IMMUNITY-D-21-00378
Description
Supplementary videos and raw data set for all the graphs shown in figures the manuscript titled "Live Imaging of SARS-CoV-2 Infection in Mice Reveals that Neutralizing Antibodies Require Fc Function for Optimal Efficacy" by Ullah et al_IMMUNITY-D-21-00378 Supplementary Video Legends: Video S1. Longitudinal Non-invasive Bioluminescence Imaging to Visualize SARS-CoV-2-nLuc Infection and Dissemination in K18-hACE2 Mice, Related to Figure 1. SARS-CoV-2-nLuc challenged mice were imaged daily in dorsal (d) and ventral (v) positions for 6 days using IVIS Spectrum to monitor virus spread in the whole body as well as neuroinvasion. Video S2. Tomographic Reconstruction of Lung Tissue Depicting SARS-CoV-2 Virions in Infected Cells, Related to Figure S2, panels B-D. Virus particles are found within membrane-enclosed exit compartments of two adjacent pulmonary capillary endothelial cells. The movie traverses the reconstructed volume to illustrate the compartments (red arrowheads) then increases in magnification to detail the virions within the compartments. Video S3. Cellular Overview and Tomogram of SARS-CoV-2 Infected Region in Lung Tissue with Alveolar Macrophages, Related to Figure S2, panels B-D. SARS-CoV-2 virions are found in regions containing identifiable immune cells. Movie begins with a large-field montaged overview, highlighting alveolar macrophages (blue), AT2 cells (green), AT1 cells (yellow) and pulmonary blood veins (red). The upper of two blood veins is detailed at higher magnification, showing 3 red blood cells (rbc) and the surrounding capillary endothelium. A region containing portions of two endothelial cells is selected for tomographic reconstruction, showing caveolae at the cell surfaces and localizing SARS-CoV-2 virions within cytoplasmic exit compartments. Video S4. Tomographic Reconstruction of SARS-CoV-2 Infected Brain Tissue Depicting Infected Neurons, Related to Figure S2, panel F. Virus particles are found within neurons, often appearing in linear groups within compartments bordering the edges of neuronal projections. The movie details the distinction between presumptive SARS-CoV-2 virions and typical synaptic neurotransmitter vesicles found in an adjacent synaptic terminal. Video S5. Tomographic Reconstruction of Mouse Testis Depicting SARS-CoV-2 Virions Within Sertoli Cells, Related to Figure S2, panel M. Virus particles are found within membrane-enclosed compartments of Sertoli cells. Additional material and structures coexist with the virions in these compartments, suggesting they may be defined as lysosomes. Presumptive SARS-CoV-2 virions can be discerned from the other structures.
Files
Steps to reproduce
Please see the Video as well as Figure Legends and Methods section for details of the experiment.