Tracing tumor evolution and subtype specification in pancreatic cancer
Data represents total raw recording of manually counted number of tdTomato+ (recombined cells) based on immunofluorescence images in islets, ducts, and acinar cell compartment in pancreas after 7 days of tamoxifen treatment in Msi2-CreER; R26-LSL-tdTomato mice (n = 3 independent mice).
Steps to reproduce
Pancreas from 3 independent biological replicates were stained and analyzed. At least 10 random high power field were imaged and recombined cells were counted to capture the overall representation of recombination event.