Single cell RNA sequencing analysis for primary human nasal epithelial cell rhinovirus infection

Published: 2 January 2026| Version 1 | DOI: 10.17632/2z5v5mvy4m.1
Contributor:
Bao Wang

Description

Human primary nasal epithelial cells were cultured at air-liquid interface (ALI) and infected with rhinovirus A1 (RV-A1), with or without IRF3 activation inhibitor BX795. Samples were collected at day1, day2 and day5 after infection for split-pool single cell RNA sequencing (Parse Biosciences). This Mendeley dataset include original code, annotation assignments and UMAP coordinates to reproduce analysis in the manuscript Wang et al. "Rhinovirus triggers distinct host responses through differential engagement of epithelial innate immune signaling". This dataset include 18235 cells from 8 samples (Mock day1/2/5, RV day1/2/5, RV+BX795 day1/2), sequenced in one batch of split-pool.

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Steps to reproduce

The original split-pool single cell RNA sequencing cell x feature matrix is provided through dbGaP. To reproduce the Seurat object, execute R codes of Analysis 1, 2 and 3. Otherwise, the original Seurat object is also provided through dbGaP. To reproduce figure 2 and 3, execute R codes of Analysis 4 and 5.

Categories

Epithelial Cell, Airway, Rhinovirus, Human Rhinovirus 1A, Single-Cell RNA Sequencing

Funders

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