Hypothermia improves neuronal network recovery in a human-derived in vitro model of oxygen-deprivation
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The data found in this repository accompanies the paper: "Hypothermia improves neuronal network recovery in a human-derived in vitro model of oxygen-deprivation". This is the data that supports the results described in the paper.
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Electrophysiology: How to Calculate Mean Firing Rate, Burst Rate, and Network Burst Rate from a Spike Train 1. Mean Firing Rate Definition: Average number of spikes (peaks) per unit of time. Formula: Mean Firing Rate (in Hz) is equal to the total number of spikes divided by the total recording time (in seconds). 2. Burst Rate Definition: Number of bursts (groups of spikes with short intervals) per unit of time. Formula: Burst Rate (in Hz) is equal to the number of bursts divided by the total recording time (in seconds). 3. Network Burst Rate Definition: Number of synchronized bursts across multiple neurons per unit of time. Formula: Network Burst Rate (in Hz) is equal to the number of network bursts divided by the total recording time (in seconds). Timestamps are in microseconds. Cell viability: a blind manual cell count was performed, distinguishing cells which were considered live when only DAPI (blue) was visible, apoptotic when positive for DAPI (blue) and Cell Event (green) and dead when positive for DAPI (blue), Cell Event (green) and PI (red). Synaptic puncta: We quantified the number of synaptic puncta by employing SynD scripts from Schmitz, Hjorth, et al. 2011 [43] using MATLAB and expressed as the number of synapsin1/2 positive puncta per 10 μm of MAP2 positive neurite.