Supplementary Figure S1 - Timeline experimental protocol - JDS19932

Published: 09-04-2021| Version 1 | DOI: 10.17632/32jrtd3dbr.1
Sanne van Gastelen


Supplementary Figure S1. Timeline of the experimental protocol per batch of calves (2 batches, 24 calves each). For each treatment (6 calves per treatment), the adaptation period ranged from 6 to 9 weeks for pair 1 to 3 (i.e., paired according to BW). The second batch of calves had a higher BW from the start, therefore having a shorter adaptation period ranging from 5 to 8 weeks. The adaptation period was followed by 7 days in climate respiration chambers (pair-housed) to measure the recovery of 13C tracers in breath after intravenous administration of [13C]sodium bicarbonate and oral administration of [1-13C]octanoate and bacterial protein, as detailed for pair 1. Subsequently, calves were fitted with harnesses to which plastic bags were attached and housed individually in a pen for 7 days, to determine the fecal excretion curves of 3 orally administrated indigestible markers (i.e., Cr-NDF, Yb2O3, and Co-EDTA), as detailed for pair 3.