Multiple Sequence Alignment: Novelty and phylogenetic affinities of a new family of tapeworms (Cestoda: Rhinebothriidea) from endangered sawfish and guitarfish

Description

A multiple sequence alignment (MSA) of 1,475 base pairs (bp) of the D1–D3 region of the 28S rRNA for the elasmobranch tapeworm order Rhinebothriidea used in a maximum likelihood (ML) phylogenetic analysis to support the establishment of a novel family within the order, as well as the description of novel species within the family. The MSA includes 75 ingroup and four outgroup sequences. Of the 75 ingroup sequences, six were newly-generated as part of this study, and are designated by "KW" or "JW" laboratory numbers. All remaining ingroup and outgroup sequences were downloaded from GenBank.

Steps to reproduce

Sequences were first trimmed to a maximum length of 1,475 bp in Geneious Prime v. 2022.0.1 (https://www.geneious.com) and then aligned using PRANK v. 170427 (Löytynoja & Goldman, 2005; Löytynoja, 2014). For PRANK, default settings were used, and the “+F” flag was not used.

Institutions

• University of Connecticut

  CT, Storrs
• University of Kansas

  KS, Lawrence

Categories

Funders

• National Science Foundation, USA

  Grant ID: DEB Nos. 0818696, 0818823, 1457762, 1457776, 1921404, and 1921411
• National Oceanic and Atmospheric Administration

  District of Columbia, United States

  Grant ID: NA16NMF4720062
• United States Fish and Wildlife Service

  District of Columbia, United States

  Grant ID: Federal Sportfish Restoration grant FL-F-F18AF00524

Licence