Multiple Sequence Alignment: Novelty and phylogenetic affinities of a new family of tapeworms (Cestoda: Rhinebothriidea) from endangered sawfish and guitarfish

Published: 22 February 2023| Version 1 | DOI: 10.17632/344bmr7ppf.1
Kaylee Herzog,


A multiple sequence alignment (MSA) of 1,475 base pairs (bp) of the D1–D3 region of the 28S rRNA for the elasmobranch tapeworm order Rhinebothriidea used in a maximum likelihood (ML) phylogenetic analysis to support the establishment of a novel family within the order, as well as the description of novel species within the family. The MSA includes 75 ingroup and four outgroup sequences. Of the 75 ingroup sequences, six were newly-generated as part of this study, and are designated by "KW" or "JW" laboratory numbers. All remaining ingroup and outgroup sequences were downloaded from GenBank.


Steps to reproduce

Sequences were first trimmed to a maximum length of 1,475 bp in Geneious Prime v. 2022.0.1 ( and then aligned using PRANK v. 170427 (Löytynoja & Goldman, 2005; Löytynoja, 2014). For PRANK, default settings were used, and the “+F” flag was not used.


University of Connecticut, University of Kansas


DNA, Tapeworm


National Science Foundation, USA

DEB Nos. 0818696, 0818823, 1457762, 1457776, 1921404, and 1921411

National Oceanic and Atmospheric Administration


U.S. Fish and Wildlife Service

Federal Sportfish Restoration grant FL-F-F18AF00524