In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

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The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(−)-limonene, sabinene, carvacrol, thymol, alpha-pinene (α-pinene), beta-pinene (β-pinene), and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs). The two highest non-toxic concentrations of each of the compounds were selected and used for testing other activities. This is a dataset related to the recently published article (Biomedicines 2023, 11(4), 1095; https://doi.org/10.3390/biomedicines11041095). Folder: “MTT” contains the raw results of three repetitions of MTT cytotoxicity assay performed after 24 hours of culture. The results of the MTT test are expressed as OD values. Folder: “LDH” contains the raw results of three repetitions of LDH proliferation assay performed after 24 and 72 hours of culture. The results of the LDH test are expressed as OD values. Folder: “Osteogenic differentiation” contains the raw results of three repetitions of osteogenic markers level (type I collagen, bone alkaline phosphatase and osteocalcin) and total cellular proteins amount after 8 days and 21 days of culture. The results of the osteogenic markers and cellular proteins tests are expressed as OD values and as concentration of the compound expressed in ng/pg per ml. Folder: “ARS” contains the raw results of three repetitions of mineral deposition assay performed after 21 days of cultures. The results of the ARS test are expressed as OD values. Folder: “ARS staining” contains raw stereoscopic images of mineral deposits in the ECM.

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