Data for: Modulation of the transcriptomic profile of the R2C tumor Leydig cell line by the adipose tissue derived hormone leptin

Published: 02-11-2018| Version 1 | DOI: 10.17632/37kjmxp6py.1
Contributors:
Luc Martin,
Pauline Roumaud

Description

We examined if leptin could alter the transcriptome of the constitutively steroidogenic rat tumor Leydig cell line R2C. These cells were treated with high levels leptin (1000 ng/ml) for 4 h, followed by mRNA extraction and RNA-Seq analysis. Total RNA extractions from cultured R2C Leydig cells were performed using the E.Z.N.A. Total RNA kit (Omega Bio-Tek, Inc., Norcross, GA). Libraries were constructed using the TruSeq Stranded mRNA LT (Illumina) for cDNA synthesis and polyA enrichment. Quality and quantification of libraries were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) and KAPA SYBR® FAST qPCR Kit (KAPA Bio Systems), respectively. Transcriptomic sequencing was performed on an Illumina NextSeq 500 in paired reads layout with approximately 13 million reads per sample and 75 bp of sequencing length. Libraries and next generation sequencing were completed by Applied Biological Materials Inc. (Richmond, BC, CAN). Paired-end raw reads are available in FASTQ format.

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