GM1 mediates the formation and maintenance of cytotoxic Aβ oligomers. Dong Yan Zhang et al.

Published: 14 July 2021| Version 1 | DOI: 10.17632/38tt768dkd.1
Contributor:
Dongyan Zhang

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Figure 2/2E.xlsx: The formation of Aβ fibrils is identified by ThT Figure 2/2F.xlsx: FRET results of GM1 and Aβ. Figure 3/3C file: The distances between atoms of each residue in wild type Aβ and atoms of all GM1 in the membrane. Figure 3/3E file: The distances between the Aβ mutant R5G and GM1 Figure 3/3G.xlsx:FRET between R5G and GM1 Figure 3/3H&I.xlsx: AβThe formation of mutant Aβ (R5G) fibrils are identified by ThT Figure 4/4B.xls: The ratio of the fluorescence intensity of Ca2+-encapsulated liposomes incubated with Aβ to that without Aβ. Figure 4/4D.xlsx: The ratio of the fluorescence intensity of dye-encapsulated liposomes incubated with Aβ to that without Aβ. Figure 5/5A-1.jpg: After purification, the molecule weights of Aβ oligomers are analyzed by mass spectrometer. Figure 5/5A-2.jpg: After purification, the molecule weights of Aβ oligomers are analyzed by mass spectrometer. Figure 5/5A-3.jpg: After purification, the molecule weights of Aβ oligomers are analyzed by mass spectrometer. Figure 5/5C: We divide the membrane Aβ oligomers into four samples according to the molecular weights. Then, we incubate the four samples with the Ca2+-encapsulated liposome and determine the fluorescence intensity. Figure 5/5D&E.xlsx: The secondary structure of the Aβ oligomers formed in the presence and absence of GM1 liposomes. Figure 5/5F&G file: The residue abundance of Aβ oligomers and fibrils formed in the presence and absence of GM1 liposomes. Figure 6/6B-1.xls: PC-12 cells incubated with and without Aβ oligomers. Cell viability is assessed by Calcein AM/ethidium homodimer-1 and calculated by the ratio of dead cells to live cells. Figure 6/6B-2.xls: PC-12 cells incubated with and without Aβ oligomers. Cell viability is assessed by Calcein AM/ethidium homodimer-1 and calculated by the ratio of dead cells to live cells.

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Institutions

Penn State

Categories

Biochemistry, Biophysics, Neurodegenerative Disorder, Alzheimer's Disease

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