PD-1 deficiency impairs eosinophil recruitment and cytotoxicity to muscle larvae of Trichinella spiralis

Published: 10 April 2024| Version 1 | DOI: 10.17632/3fbzppcfbx.1
Yuli Cheng


Blockade of PD-1 may serve as a promising strategy in controlling pathogen infections through enhancing host immune cell function. Eosinophils are the essential components of the type 2 immune mechanism that function in host defense against helminth infections. Here we examine the role of PD-1 in eosinophilia in mice during T. spiralis infection. Unexpectedly, PD-1 deficient (PD-1-/-) mice developed higher muscle larvae loads and exacerbated disease compared to wild type mice. Further study showed that PD-1 deficiency impaired the recruitment of eosinophils into the parasite invaded tissue and their cytotoxicity to muscle larvae through reduced production of eosinophil-specific chemokines, and expression of adhesion molecules intergrin α4β7 and L-selectin on eosinophils and VCAM-1 on vascular endothelial cells after T. spiralis infection. The compromised T-helper 2 (Th2) cytokine response contributed to the impaired adhesion interactions involved in eosinophil migration in PD-1-/- mice infected with T. spiralis. Our data revealed the positive role of PD-1 in host defense against helminth infection by regulating the migration and trafficking of eosinophils to the infection site.



Capital Medical University


Parasite Immunology


National Natural Science Foundation of China


Natural Science Foundation of Beijing Municipality


Natural Science Foundation of Beijing Municipality