IRI-SKEM-EGCG

Description

Since oxidative stress and neutrophil infiltration play a central role in ischemia-reperfusion injury (IRI), the effects of epigallocatechin gallate (EGCG) on neutrophil infiltration-related markers were investigated. The IRI model utilizing an elastic rubber band was 4 + 2 h (IRI group). In sham-control (SC) group, all steps but band binding were preserved. Saline (10 mL/kg/i.p.) and EGCG (25 and 50 mg/kg/i.p.) were administered 30 minutes before the reperfusion (IRI-SF, IRI-25, and IRI-50 groups). Creatine phosphokinase (CPK), lactate dehydrogenase (LDH), total oxidant status (TOS), total antioxidant status (TAS), myeloperoxidase (MPO), E-selectin, P-selectin, L-selectin, intercellular adhesion molecule-1 (ICAM-1), and various cytokines (IL-1β, IL-6, TNF-) were determined in serum and gastrocnemius muscle samples. CPK, LDH, and TOS levels were higher in the IRI group compared to the SC group; insignificant decreases were observed in the IRI-50 group compared to the IRI group. Regarding TAS, a visible decrease was seen in the IRI compared to SC group, which was significantly increased by 50 mg/kg of EGCG. Regarding remaining parameters, statistically insignificant increases in IRI compared to SC and decreases in the EGCG-treated versus IRI groups were detected. We claim that EGCG might have a beneficial effect via an interaction with neutrophil infiltration-related markers.

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