PUFA overnutrition - Liver and Adipose Tissue Paper 2021
Description
Data used for the elaboration of the scientific article referring to body characteristics, plasma profile, metabolic activity and the hepatic anti-oxidant system, oxidative damage and inflammatory profile of adipose tissue. The animals used in the present study are offspring of rats that received excessive supplementation with polyunsaturated fatty acids from the omega 3 and and 6 families throughout pregnancy and lactation.
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Females’ rats were split into 3 experimental groups: control (C), supplemented with fish oil (FO group), or soybean oil (SO group). The FO used was a mixed marine triacylglycerol preparation containing 180 g eicosapentaenoic acid (EPA) and 120 g docosahexaenoic acid (DHA) per kg, purchased from Vitamins Sundown Naturals© (Recife, Pernambuco, Brazil). The SO used was composed primarily of triacylglycerol with 50% of linoleic acid purchased from Bunge© (Luziânia, Goiás, Brazil). The oils were supplemented at a level of 4 g/kg b.w. per day and were provided as a single bolus using a pipette during 21 days before and during mating, gestation, and lactation periods, making a total of 68 ± 5 days. At weaning (21 days), male offspring were housed in groups of four animals and maintained until the end of the experiment. At 60 days old, the animals were killed by decapitation, blood collected, and plasma obtained by centrifugation. Bodyweight, nasal-anal length, liver weight, mesenteric, and retroperitoneal fat pad mass were measured. The liver and the adipose tissues were used to evaluate energy metabolism and oxidative stress biomarkers.