No toxicity to the tropical marine microalgae Tisochrysis lutea from gamma radiation
Description
Raw data obtained in 72 h chronic toxicity tests exposing microalgae to gamma radiation and nickel as a reference toxicant following standard methods from OECD Guideline Test 201, and the code used to analyse data in R Studio using Bayesnec package.
Files
Steps to reproduce
1. Culture microalgae under axenic conditions and establish when they're in the growth phase using a flow cytometer by measure the cell density of a fresh culture every day. Graph the data to visualise when the exponential growth phase is, usually day 5-6. 2. Reference toxicant tests and optimise methods 3. Radiation exposure tests Please see the manuscript, OECD Guideline Test 201 and McKnight et al. 2023 for comprehensive test methods. 4. Pool the data together, used code to run Bayesnec modelling and visualise the data.