Systematic Quantification of Population Cell Death Kinetics in Mammalian Cells

Published: 30 April 2017| Version 1 | DOI: 10.17632/3pnv5wh5jm.1
Scott Dixon


These files and associated data are associated with the forthcoming publication by Forcina et al in Cell Systems. This work uses scalable time-lapse analysis of cell death kinetics (STACK) to profile cell death kinetic in U-2 OS osteosarcoma cells and T98G glioblastoma cells expressing nuclear-localized mKate2 and treated with 1,833 different bioactive compounds, including approved drugs, investigational anti-cancer agents and natural products, at a fixed concentration of 5 μM. The first file contains details about the 1,833-member bioactive compound library. The three subsequent files contain raw counts for live (mKate2+) and dead (SG+) cells from library-treated cells and control (DMSO)-treated populations. Computed lethal fraction scores are also shown. Each result file contains all raw data in the first tab, as well as a second tab with only those data that passed the quality control (QC) measures described in the Methods section of this work.



Stanford University


Health Sciences, Cell Biology, Chemotherapy, High-Throughput Screening, Tumor Cell Biology