Resting state fMRI data from n=9/19 mice under medetomidine-isoflurane anesthesia (mice were first deeply anesthetized with isoflurane 4% induction, intubated and artificial ventilated 90 BPM, then switched to halothane 0.75%) from Gutierrez-Barragan et al. 2021 (DOI pending). Scans were acquired at the IIT laboratory in Rovereto (Italy) on a 7.0 Tesla MRI scanner (Bruker Biospin, Ettlingen) with a BGA-9 gradient set, a 72 mm birdcage transmit coil, and a four-channel solenoid receive coil. Awake scans used a single-shot echo planar imaging (EPI) sequence with the following parameters: TR/TE=1200/15 ms, flip angle=60°, matrix=100 x 100, 24 coronal slices (voxel-size 200 x 200 x 500 µm), for a total of 1600 time points, total acquisition time of 32 minutes. It is suggested to remove the first 100 scans (2 minutes) to account for thermal gradient equilibration.
Steps to reproduce
Please follow the preprocessing and analysis methods in Gutierrez-Barragan et al., 2021 (DOI pending) and use the software listed in the KEY RESOURCES TABLE.