Dataset on porcine retinal cells after the optic nerve transection.

Published: 16-10-2020| Version 1 | DOI: 10.17632/3v9cmw4vn4.1
Contributor:
Ginga Ito

Description

Figure 1. Retinal morphology and apoptosis after organ culture of porcine retinas. (A) Histological evaluation of cultured retinas. Porcine retinas were cultured in Dulbecco’s modified Eagle’s medium for the indicated times. Cell nuclei were stained with hematoxylin and the cytoplasm with eosin. Scale bar = 50 μm. (B) TUNEL assay of retinal sections after organ culture. TUNEL-positive cells are shown in green. Nuclei are stained blue with 4'-6-diamidino-2-phenylindole (DAPI). As a positive control, retinas were cultured in the presence of 5 mM sodium glutamate for 12 h. Scale bar = 50 μm. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Figure 2. Immunohistochemistry of retinal cell markers after organ culture. Antibodies against rhodopsin (green, panels a-d), M-opsin (red, panels e-h), NeuN (green, panels i-l), glutamine synthetase (red, panels m-p), and GFAP (red, panels q-t) were used. Nuclei are stained blue with DAPI. Scale bar = 50 μm. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; NeuN, neuronal nuclei; GFAP, glial fibrillary acidic protein.