LC-MS/MS data for IseG, SauS, SauC, SauD
Published: 22 May 2023| Version 1 | DOI: 10.17632/3x7g6hr63v.1
Protein identification by Mass Spectrometry.
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Cells were harvested by centrifugation, lysed by boiling in Laemmli loading buffer, and analyzed on a 10% SDS/PAGE gel. Prominent protein bands induced by growth on sulfonate substrates were manually excised. After in-gel digestion and extraction, the peptide mixtures were loaded onto Orbitrap Fusion LUMOS MS. The MS/MS spectra from each LC-MS/MS run were searched against the B. wadsworthia protein database GCF_000185705.2 (Bilo_- wads_3_1_6_V2) from UniProt (release date of Mar, 2022; 68,406 entries) using an in-house Proteome Discoverer (Version PD 2.2, Thermo-Fisher Scientific). Protein identifications were performed based on Sequest HT.
Liquid Chromatography Mass Spectrometry