Raw data files from RNA seq on HCC827 NSCLC cells

Published: 12-08-2019| Version 1 | DOI: 10.17632/3xh9r2yj3c.1
qian liang


RNA was subjected to RNA-Seq analysison BGISEQ-500 system by Beijing Genomics Institute (BGI), China. Besides, the RNA was sheared and reverse transcribed through random primers to get cDNA used for library construction. Subsequently, perform sequencing on prepared library(Huang et al., 2017).All the generated raw sequencing reads were filtered to getclean reads stored as FASTQ format(Cock et al., 2010). Bowtie2 and HISAT was used to map clean reads to reference gene and genome repectively (Kim et al., 2015; Langmead et al., 2009). RSEMwas used to quantified the Genes expression level (FPKM)(Li and Dewey, 2011). NOISeq method was used to screen out differentially expressed genes between two groups with Foldchange≥2 and divergeprobability ≥0.8 (Tarazona et al., 2011). Gene Ontology (GO) and pathway annotation and enrichment analyses were based on the Gene Ontology Database (http://www.geneontology.org/) and KEGG pathway database (http://www.genome.jp/kegg/), respectively.