Raw Data-Manuscript_The deubiquitinase OTUD1 negatively regulates inflammation and plays an immunoregulatory role in sepsis-induced lung injury

Published: 2 September 2022| Version 1 | DOI: 10.17632/3z2tr4zjvf.1
Contributor:
Tingqian Ming

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These additional files are uploaded as the "Raw Data of Original Materials", which we would like to share for review purpose only. Document List for all the Raw data: Raw data 1: Demographics and Clinical characterization of patients; Main clinical indexes and inflammatory biomarkers in sepsis patients. Raw data 2: The Detailed Transcriptomics profile of clinical sepsis patients; Public array-based profiling of sepsis patients from the GEO database of NCBI: GSE57065; GSE28750; GSE76293. Raw data 3: The Detailed Proteomics profile and primary bioinformatics analysis of OTUD1 KO mice. Raw data 4: The Detailed Transcriptomics profile and primary bioinformatics analysis of CLP-induced experimental sepsis in OTUD1 KO mice. Raw data 5: The original images for HE staining and Transmission electron microscopy;lung injury scores; lung W/D ratio;MPO, SOD and LDH activities;PaO2+PaO2/FiO2 ratio;survival rates. Raw data 6: The levels of inflammatory cytokines in the serum of mice (ELISA):TNF-α, IL-6, IL-1β and HMGB1 levels. Raw data 7: The levels of inflammatory cytokines of LPS-stimulated BMDMs (qRT-PCR-1):TNF‐α, IL-6, IL‐1β and HMGB1 expression levels; The levels of inflammatory cytokines of LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (qRT-PCR-2):TNF‐α, IL-6, IL‐1β and HMGB1 expression levels. Raw data 8 The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs (Western Blot-1):Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (Western Blot-2):Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in lung tissues of mice (Western Blot-3):Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins.

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