Raw Data-Manuscript_The deubiquitinase OTUD1 negatively regulates inflammation and plays an immunoregulatory role in sepsis-induced lung injury
Description
These additional files are uploaded as the "Raw Data of Original Materials", which we would like to share for review purpose only. Document List for all the Raw data: Raw data 1: Demographics and Clinical characterization of patients; Main clinical indexes and inflammatory biomarkers in sepsis patients. Raw data 2: The Detailed Transcriptomics profile of clinical sepsis patients; Public array-based profiling of sepsis patients from the GEO database of NCBI: GSE57065; GSE28750; GSE76293. Raw data 3： The Detailed Proteomics profile and primary bioinformatics analysis of OTUD1 KO mice. Raw data 4： The Detailed Transcriptomics profile and primary bioinformatics analysis of CLP-induced experimental sepsis in OTUD1 KO mice. Raw data 5： The original images for HE staining and Transmission electron microscopy；lung injury scores; lung W/D ratio；MPO, SOD and LDH activities；PaO2+PaO2/FiO2 ratio；survival rates. Raw data 6： The levels of inflammatory cytokines in the serum of mice (ELISA)：TNF-α, IL-6, IL-1β and HMGB1 levels. Raw data 7: The levels of inflammatory cytokines of LPS-stimulated BMDMs (qRT-PCR-1)：TNF‐α, IL-6, IL‐1β and HMGB1 expression levels; The levels of inflammatory cytokines of LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (qRT-PCR-2)：TNF‐α, IL-6, IL‐1β and HMGB1 expression levels. Raw data 8 The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs (Western Blot-1)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in LPS-stimulated BMDMs by pretreatment with 5Z-7-ox (Western Blot-2)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins; The activation of TAK1-MAPK/NF-κB pathways in lung tissues of mice (Western Blot-3)：Expression levels of TAK1, p38, JNK, ERK, p65 as well as their respective phosphorylated proteins.