Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
Description
This dataset contains a subset of raw images from the published work titled "Imaging the time course of DNA damage response at a nonrepetitive endogenous locus" in Cell Reports Methods. The raw images are organized into zip folders according to the figure they correspond to in the manuscript. All images are in .nd2 format and consist of z-stack acquisitions with a step size of 0.35 μm. Most images feature U2OS cells. If RPE1 cells were used (Figure 4), this is indicated in the file name. HEK293T cells were used in Figure 2 to showcase fixation conditions with H2B staining. U2OS cells were used in Figure 2 with etoposide treatment. Each file represents a specific fixation time point following either vfCRISPR activation or electroporation with CRISPR-Cas9, denoted by labels such as 5m (5 minutes), 3h (3 hours), NL (No Light, indicating no vfCRISPR activation), NoEP (No Electroporation), or EP (Electroporation with CRISPR-Cas9). Labeling details include: ACTB and MUC4 loci - Cy5; 53BP1 and H2B - Alexa 555 (A555); γH2AX and BRCA1 - Alexa 488 (A488); Hoechst (DAPI). Fixation conditions in the Figure 2 folder are labeled as: M100 - Methanol; MAA - Methanol:Acetic Acid; M100toMAA - Methanol followed by Methanol:Acetic Acid. Etoposide treatment conditions are denoted as Control (DMSO) or concentrations ranging from 1-100 μM Etoposide. G1 and G2 synchronized U2OS cells are labeled accordingly in the folder for Figure 4.