Extended Figure1
Description
Unprocessed Western blot images of anti-MECOM staining, showing expression of three MECOM isoforms (MDS1/EVI1, EVI1 and EVI1Δ) in the wild type (WT) control line. Anti-alpha-tubulin, anti-beta-actin and Coomassie brilliant blue staining used as loading control. - WTC-RFP EC (+ctrl): ETV2-overexpressed endothelial cells in WTC stem cell background, not used in other experiments - 409b2 wt (WT): 409b2 wt organoid, non-edited, control - A2: 409b2 organoid, not full KO, not used in other experiments - B1-17 (KO1): 409b2 organoid, full KO, referred to as KO1 - D2-22 (KO2): 409b2 organoid, full KO, referred to as KO2 - G8-1: 409b2 organoid, not full KO, not used in other experiments Related to Figure S7, Characterization of MECOM knock-out hBVOs.