supplementary material
Description
the supplementary material of the "Network pharmacology integrated with experimental validation uncover that Secoisolariciresinol Diglucoside ameliorate premature ovarian insufficiency via PI3K/Akt pathway"
Files
Steps to reproduce
1. Identification of Potential SDG Targets The Simplified Molecular-Input Line-Entry System (SMILES) notation and three-dimensional structure of SDG were obtained from the PubChem database (https://pubchem.ncbi.nlm.nih.gov/). Potential targets were identified using resources such as PharmMapper (https://lilabecust.cn/pharmmapper/), SwissTargetPrediction (http://www.swisstargetprediction.ch/), TargetNet (http://targetnet.scbdd.com/), and the Comparative Toxicogenomics Database (CTD, https://ctdbase.org/). 2. Identification of POI-Related Targets Employing the DisGeNET database (https://www.disgenet.org/) and GeneCards repository (https://www.genecards.org/), we identified genetic targets associated with Premature ovarian insufficiency. To bolster the reliability of our findings, only POI-related targets exhibiting a gene-disease association score of 0.1 or higher in DisGeNET, and those surpassing a relevance score threshold of 20 in GeneCards, were considered.