The data of study about the chitosan-selenium nanoparticles on depression-like behaviour induced by fluoride in mice via JAK2-STAT3 pathway
These data showed the morphology, product composition and crystal structure of CS-SeNPs. Data1 found that the distribution frequency of CS-SeNPs with a particle size of 41.5-48.8 nm was the highest, namely 19.48%. Data 1 also demonstrated that the diameter of manufactured CS-SeNPs was all less than 100 nm, in line with the preparation requirements of nanomaterials. Data 2 showed the maximum absorption of the CS-SeNPs system was at 245 nm, and there was absorption in the visible region of 300~600 nm, which indicated that the prepared CS-SeNPs are different from the simple mixing of solution, that is, the new substance CS-SeNPs is generated. Moreover, Data 3 of Raman spectrum analysis showed one resonance peak of CS-SeNPs was identified at 246 cm−1 depicted monoclinic Se (m-Se). However, there is no signal absorption peak around 234cm-1, or the signal of absorption peak is very weak, indicating that there is no trigonal Se (t-Se) or the amount of it is very small. In addition, Data 4 and 5 claimed that CS-SeNPs were able to clear •DPPH and •ABTS in a dose-dependent manner, and their capacity to scavenging radicals was stronger than that of Vc, which was equal to approximately 8 times (•DPPH) or 20 times (•ABTS) of that of Vc, was acceptable. These data could be used for the These data can be used to understand the physical and chemical and biological characteristics of the materials prepared in this study of CS-SeNPs. It can also be compared with nanomaterials prepared by other methods. Furthermore, it was revealed that it had excellent antioxidant activity to provide data support.
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The following was the data gathered method: Raw data 1: The data came from Nano Measure 1.2 software. In this software, we opened the TEM image and measured the particle diameter. Then, a total of three local images of three samples were selected for statistics. Raw data 2: The ultraviolet-visible (UV-Vis) absorption spectra of different reaction liquids were scanned with an ultraviolet (UV) absorption spectrometer (UH5300, Shanghai) in the wavelength range of 190-1100 nm. Deionized water was used as the reference solution, and the data interval was set to 5.0 nm. Dilute CS, CS+Vc, H2SeO3, Vc, H2SeO3+Vc, and CS-SeNPs to appropriate proportions, respectively, and scan the spectrum at the key laboratory of chemical biology and molecular engineering of the ministry of education of Shanxi University. Finally, all the above data are plotted in the GraphPad Prism 6.01 software. Raw data 3: Use a Raman spectrometer (HORIBA E volution, LabRAM, USA) to characterize the crystal structure of CS-SeNPs. Connect the LabRAM HR E volution Raman analyzer to the computer, open the software Raman Analyzer, turn the laser lock switch clockwise to turn on the laser, and warm up for 30 min. The sample was placed on the glass slide, and the parameters were set according to the excitation wavelength of 532 nm and the excitation power of 0.2 milliwatts. The distance between the moving scanner and the sample slide was adjusted to 2 mm. Get the following scan image and obtain the raw data. The original data is generated on the Orign software and corrected to produce the final image. Raw data 4: According to the instructions of the DPPH free radical scavenging ability test kit (Nanjing Jiancheng Bioengineering Institute), the CS-SeNPs solution was diluted in multiple proportions and then operated in a cuvette. At the same time, use Vc as a control. The DPPH scanning ability was calculated according to the measured results: DPPH scanning ability(%) =[(1 – (Asample − Acontrol)÷Ablank)×100]%. Half-maximum effect concentration (EC50) was calculated by the GraphPad Prism 6.01 software. Raw data 5: According to the instructions of the ABTS free radical scavenging ability test kit (Nanjing Jiancheng Bioengineering Institute), the CS-SeNPs solution was diluted in multiple proportions and then operated in a cuvette. At the same time, use Vc as a control. The ABTS scanning ability was calculated according to the measured results: ABTS scanning ability(%) =[Ablank−(Asample−Acontrol)]÷Ablank×100%. Half-maximum effect concentration (EC50) was calculated by the GraphPad Prism 6.01 software.