Supplementing high-fibre olive pomace and multi-enzyme to broiler chicken's diet can improve health and performance

Published: 2 April 2024| Version 1 | DOI: 10.17632/42wp56n5kh.1
Osama Ghaffar,
, Sarwar Aziz,
, Karzan Saleh,


This study assessed the effects of high-fibre olive pomace (OP) and exogenous multi-enzyme (phytase, cellulase, and xylanase) on broiler chickens' growth performance, internal organs, intestinal morphology, blood traits, lipid profile of serum, and health. 300 one-day-old Ross 308 chicks were randomly divided into six groups using a 2 × 3 factorial design with 0 and 0.5g/kg multi-enzyme and 0, 5, and 10% olive pomace. The diet was primarily based on corn and soybean meal. The experiment was carried out in three stages: starter, grower, and finisher. The results indicated that the inclusion of OP at both 5% and 10% levels did not negatively impact broilers' performance (P>0.05). However, supplementing the diet with multi-enzyme significantly increased feed consumption, body weight, and weight gain (P<0.05). Furthermore, chickens offered with 10% OP + multi-enzyme exhibited the best performance compared to other experimental groups. The mortality rate also showed a non-significant decline of almost 5% (P>0.05). Additionally, administration of OP and multi-enzyme or a combination of them to the broilers' diet improved serum lipid profile and liver enzyme activity (P<0.05) and did not affect the relative weight and length of internal organs, and intestinal histomorphology (P>0.05). In conclusion, simultaneous use of multi-enzyme and diet containing olive pomace could improve the serum lipid profile, liver enzyme activity, and overall health without adverse effect on broilers performance.



University of Raparin


Poultry Nutrition, Poultry Physiology